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anti-LRP1 (85kD) mouse monoclonal, 5A6, lyophilized w/o sodium azide, purified

The antibody recognizes human 85 kD light chain of LRP. Cross-reacts with rabbit LDL receptor-related protein (LRP) or mouse LRP.
Cat. No.: 61066
Quantity:  50 µg
No. of experiments:  ≤ 400 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone 5A6
Immunogen human LRP/alpha2MR
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity hamster, human, mouse, rabbit, rat


Tested applications Tested dilutions
Flow Cytometry (FACS) assay dependent
Immunohistochemistry (IHC) - frozen 1:20
Immunohistochemistry (IHC) - paraffin 1:20 (microwave treatment recommended)
Western Blot (WB) assay dependent



5A6 specifically reacts with the 85 kD b-chain of human LRP/a2MR which is expressed in a restricted spectrum of cell types (for a recent review cf. Herz & Strickland). A strong immunohistochemical reaction is seen in hepatocytes, tissue macrophages, subsets of neurones and astrocytes in the central nervous system, fibroblasts, smooth muscle cells, and monocyte-derived foam cells in atherosclerotic lesions in the arterial wall. The antibody can also be used for the characterization of a subset of myelo-monocytic subtypes of chronic and acute leukemia (CD 91). Neutralizing effects of 5A6 have also been reported (Basu et al., 2001).

Basu S, Binder RJ, Ramalingam T, Srivastave PK: CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp 70, and calreticulin. Immunity 14, 303-313 (2001)


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