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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2b |
| Clone | 5A6 |
| Immunogen | human LRP/alpha2MR |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
| Intended use | Research use only |
| Tested species reactivity | hamster, human, mouse, rabbit, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Flow Cytometry (FACS) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:20 |
| Immunohistochemistry (IHC) - paraffin | 1:20 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
5A6 specifically reacts with the 85 kD b-chain of human LRP/a2MR which is expressed in a restricted spectrum of cell types (for a recent review cf. Herz & Strickland). A strong immunohistochemical reaction is seen in hepatocytes, tissue macrophages, subsets of neurones and astrocytes in the central nervous system, fibroblasts, smooth muscle cells, and monocyte-derived foam cells in atherosclerotic lesions in the arterial wall. The antibody can also be used for the characterization of a subset of myelo-monocytic subtypes of chronic and acute leukemia (CD 91). Neutralizing effects of 5A6 have also been reported (Basu et al., 2001).
Basu S, Binder RJ, Ramalingam T, Srivastave PK: CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp 70, and calreticulin. Immunity 14, 303-313 (2001)
Reference
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