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anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2, Biotin Conjugate

The antibody binds to pVIII major coat protein of filamentous phages of the fd, F1 and M13 group. Suitable for the identification of recombinant phages in antigen and antibody-phage systems and phage ELISA.
Cat. No.: 61597
Quantity:  250 µL (100 µg/mL)

Delivery Time: usually 1-7 working days

€222.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone B62-FE2
Immunogen fd phages from E. coli F+ strain (JM109)
Purification protein A affinity chromatography
Conjugate biotin
Formulation PBS with 0.5% BSA and 0.09% sodium azide
Storage store at 2 – 8 °C
Tested species reactivity filamentous phages F1, filamentous phages fd, filamentous phages M13
Note centrifuge prior to opening
Intended use Research use only

Applications

Tested applications Tested dilutions
ELISA 1:5,000 – 1:10,000
Phage Display immunoassays for the identification of recombinant antigen- or antibody-phages; Detection limit: 10^7 phage particles
Western Blot (WB) assay dependent

Background

Details

Specificity: B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (Kneissel et al., 1999).

Kneissel, S. et al. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21–8 (1999).

Reference

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