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anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2, lyophilized, purified, 100 µg

The antibody binds to pVIII major coat protein of filamentous phages of the fd, F1 and M13 group. Suitable for the identification of recombinant phages in antigen and antibody-phage display systems and phage ELISA.
Cat. No.: 61097
Quantity:  100 µg

Delivery Time: usually 1-7 working days

€165.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone B62-FE2
Immunogen fd phages from E. coli F+ strain (JM109)
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity filamentous phages F1, filamentous phages fd, filamentous phages M13

Applications

Tested applications Tested dilutions
ELISA 1:1000
Immuno Electron Microscopy (IEM) assay dependent
Phage Display assay dependent (detection limit: 10^7 phage particles)
Western Blot (WB) assay dependent

Background

Details

B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (Kneissel et al. 1999)

Kneissel, S. et al. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21–8 (1999).

Reference

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