anti-mTOR rabbit polyclonal, purified, serum
- Rabbit polyclonal
- Suitable for IHC, ICC/IF, IP and WB
- Reacts with human, mouse, dog, monkey and rat
- Knock out validated
|Application||ICC/IF, IHC, IP, WB|
|Reactivity||Dog, Human, Monkey, Mouse, Rat|
|Storage||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Immunogen||Peptide from human mTOR (GTTVPESIHSFIGDGLVKPE)|
|Formulation||PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA|
|Synonym||Mechanistic target of rapamycin, Mammalian target of rapamycin
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:500-1:2,000|
|Immunohistochemistry (IHC) - paraffin||1:50-1:100 (microwave treatment recommended)|
|Immunoprecipitation (IP)||10 µg|
|Western Blot (WB)||1:500-1:2,000|
Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in protein synthesis, cell growth, proliferation and differentiation. It is inhibited by rapamycin, which has immunosuppressant functions. The 290 kDa protein is highly regulated by growth factors and nutrients like glucose and amino acids. mTOR is involved in several pathways from protein degradation via autophagy to protein synthesis via translation initiation (4EBP), ribosome and lipid synthesis. It is located in vesicles and the cytosol.
Q & A's
0 of 0 reviews
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with hamster, human, mouse, rabbit and rat
- Isotype: IgG2b
- Set provides anti-p62 antibody and associated western blot control - positively tested PLC cell lysate.
- The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy.
- Mouse monoclonal
- Suitable for FACS, ICC/IF, IHC and WB
- Reacts with cat, dog, goat, guinea pig, horse, human, monkey, pig, rabbit and rat
- Isotype: IgG1 kappa