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WB with anti-Vimentin antibody (Cat. No. 61013, 1:500) and secondary anti-mouse HRP antibody (Cat. No. 90002, 1:25,000), sample SV80 whole cell lysate (5-10 µg) Zoom

WB with anti-Vimentin antibody (Cat. No. 61013, 1:500) and secondary anti-mouse HRP antibody (Cat. No. 90002, 1:25,000), sample SV80 whole cell lysate (5-10 µg)

anti-mouse IgG goat polyclonal, HRP conjugate

Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Cat. No.: 90002
Quantity:  100 µg

Delivery Time: usually 1-7 working days

€87.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host goat
Antibody Type polyclonal
Immunogen Gamma Immunoglobin Fc region
Purification affinity chromatography
Conjugate peroxidase/ HRP
Formulation lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity mouse

Applications

Tested applications Tested dilutions
ELISA 1:1,250 - 1:25,000
Western Blot (WB) 1:2,500 - 1:50,000

Background

Details

Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

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