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anti-Myosin mouse monoclonal, 414.0, supernatant

Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasmIn Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain), no reaction with light chains. It is not reactive with alpha-cardiac myosin.
Cat. No.: 10605
Quantity:  1 mL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$331.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone 414.0
Immunogen chicken skeletal muscle cell preparation
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity chicken, human, mouse, rabbit

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5 - 1:10
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain), no reaction with light chains.
It is not reactive with alpha-cardiac myosin.

Positive control: muscle, myofibrils (chicken)

Reference

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