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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgM |
| Clone | 414.0 |
| Immunogen | chicken skeletal muscle cell preparation |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | chicken, human, mouse, rabbit |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:5 - 1:10 |
| Immunohistochemistry (IHC) - frozen | 1:5 - 1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5 - 1:10 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain), no reaction with light chains.
It is not reactive with alpha-cardiac myosin.
Positive control: muscle, myofibrils (chicken)
Reference
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