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anti-Myozap mouse monoclonal, 517.67, supernatant

The anti-myozap monoclonal antibody detects myozap (myocardium zonula adherens protein), a major 54 kD polypeptide present in myocardial junctions (composite junctions in the intercalated disks), also localized in adherens type junctions of the entire cardiovascular system and in endothelia of the blood and lymph vascular systems.
Cat. No.: 651169
Quantity:  5 mL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$278.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 517.67
Immunogen Synthetic peptide of human myozap (myocardium zonula adherens protein)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity bovine, human, mouse, pig, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) ready-to-use
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment with 100 mM Tris pH 9.5, 5% urea buffer; 10 min at 120°C recommended)
Immuno Electron Microscopy (IEM) assay dependent
Immunoprecipitation (IP) assay dependent
Western Blot (WB) 1:10 - 1:50

Background

Details

Detects myozap (myocardium zonula adherens protein), a major 54 kD polypeptide present in myocardial junctions (composite junctions in the intercalated disks), also localized in adherens type junctions of the entire cardiovascular system and in endothelia of the blood and lymph vascular systems.
Reactivity on cultured cell lines: HL-1, HUVEC.
Positive control: myocard.

Reference

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