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Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | Synthetic peptide of mouse Nephrin (intracellular domain, aa1243-1256, EPGSLPFELRGHLV) |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 100 (IHC, 50 µl/slide); ≤ 10 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | human, mouse |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:50 |
Immunohistochemistry (IHC) - paraffin | 1:50 (microwave treatment recommended) |
Western Blot (WB) | 1:500 |
Background
Details
The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE).
Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Immunolocalization: The antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.
Tested cultured cell lines: PCL (podocyte cell line), M-1 (cortical collecting duct cells)
Reference
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Species
Application
mouse
IHC-IF (paraffin)
mouse
IHC-IF (paraffin)
mouse
IHC-IF (paraffin)
mouse
IHC-IF (paraffin)
mouse
IHC-IF (frozen)
human
IHC-IF (frozen)
mouse
IHC (paraffin)
human
mouse
rat
mouse
rat
IHC (paraffin)
mouse
IHC (paraffin)
mouse
IHC-IF (frozen)
mouse
rat
rat
IHC-IF (paraffin)
mouse
IHC-IF (paraffin)
mouse
IHC-IF (paraffin)
grivet monkey
WB
mouse
IHC (paraffin)
mouse
IHC-IF (frozen)
human
IHC-IF (paraffin)
human
mouse
mouse
IHC-IF (paraffin)
human
IHC-IF (paraffin)
human
mouse
mouse
IHC-IF (paraffin)
human
IHC-IF (paraffin)
mouse
whole mount
mouse
IHC (paraffin)
human
IHC
ICC-IF
FACS
ICC-IF
FACS