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mouse kidney (courtesy of J.Heß, University Hospital Heidelberg) Zoom

mouse kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Nephrin guinea pig polyclonal, serum

The anti-nephrin antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein, and stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas. Directed against the intracellular domain.

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Cat. No.: GP-N2
Quantity:  100 µL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide); ≤ 10 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$312.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Synthetic peptide of mouse Nephrin (intracellular domain, aa1243-1256, EPGSLPFELRGHLV)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide); ≤ 10 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:50
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:500

Background

Details

The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE).
Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Immunolocalization: The antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.
Tested cultured cell lines: PCL (podocyte cell line), M-1 (cortical collecting duct cells)

Reference