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anti-Non-Filamentous Actin mouse monoclonal, 1C7, supernatant

The antibody detects a discrete form of actin in the nucleus, which is different from the nuclear actin revealed by antibody 2G2 (cat. no. 651132). Reactive epitope: TERGYSFVTT
Cat. No.: 651154
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€244.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 1C7
Immunogen chemically cross-linked actin dimmer
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent (only after non-denaturing conditions)

Background

Details

Specific for non-filamentous actin in cells (Schoenenberger et al., 2005). In addition the antibody detects a discrete form of actin in the nucleus, which is different from the nuclear actin revealed by antibody 2G2 (cat. no. 651132).
Reactive epitope: TERGYSFVTT.

Reactivity on cultured cell lines:  Rat fibroblasts, HeLa

Schoenenberger CA, Buchmeier S, Boerries M, Suetterlin R, Aebi U, Jockusch BM: conformation-specific antibodies reveal distinct actin structures in the nucleus and the cytoplasm. J Struct Biol 152, 157-168 (2005).

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