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anti-Nucleolar Protein NO38 mouse monoclonal, No-185, supernatant concentrate

The antibody recognizes a 38kD nucleolar protein (NO38) in Xenopus (also termed numatrin, B23, nucleophosmin, in other species). NO-185 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
Cat. No.: 652125
Quantity:  250 µL

Delivery Time: usually 1-7 working days

$339.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone No-185
Immunogen Nucleolar fraction prepared from Xenopus laevis oocytes
Purification hybridoma cell culture supernatant concentrate
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity chicken, Pleurodelis, Triturus, xenopus (negative in higher animals)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:300 - 1:500 (fixation with paraformaldehyde recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) 1:1,000 - 1:2,000

Background

Details

The antibody recognizes a 38 kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species).
No-185 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
The epitope recognized is different from the one recognized by clone No-63 (cat.no. 612123).

Reference

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