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anti-Nucleolar Protein NO38 mouse monoclonal, No-63, supernatant

Recognizes a 38kD nucleolar protein (NO38) in Xenopus (also termed numatrin, B23, nucleophosmin, in other species). No-63 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
Cat. No.: 651123
Quantity:  5 mL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$245.00
Excl. VAT, excl. Shipping Cost

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Cat. No. Product Name Format Quantity Price
612123

anti-Nucleolar Protein NO38 mouse monoclonal, No-63

ascites fluid 250 µL
$271.00
Excl. VAT, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone No-63
Immunogen nucleolar fraction prepared from Xenopus laevis oocytes
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity chicken, Pleurodelis, Triturus, xenopus

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use (also after fixation with paraformaldehyde)
Immunoprecipitation (IP) assay dependent (from Xenopus oocyte extracts nucleoplasmin - a 30 kD protein - is co-precipitated, which shows high sequence homology to NO38)
Western Blot (WB) 1:10 - 1:20 (for blotting transfer nitrocellulose sheets are superior to nylon sheets)

Background

Details

Recognizes a 38kD nucleolar protein (NO38) in Xenopus (also termed numatrin, B23, nucleophosmin, in other species). No-63 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.

Reference

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