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anti-Nucleolus-Organizing Region mouse monoclonal, X-11 + X-19, supernatant

The mabs represent marker for nucleolar organizing region (NOR) and reacts with treacle protein (described as TCOF1).
Cat. No.: 65148
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone X-11 + X-19
Immunogen nuclear pore complex-lamina fraction of XLKE-A6 cells (cultured cell line derived from Xenopus laevis)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity Xenopus laevis (negative with human, chicken)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The mabs represent marker for nucleolar organizer region (NOR), putatively staining the protein treacle (described as TCOF1).

Polypeptides reacting: Mr 240 kD band in SDS gels and lower molecular weight bands (e.g. 160 kD band, Gonzales et al. 2005).

Gonzales B, Yang H, Henning D, Valdez BC: Cloning and functional characterization of the Xenopus orthologue of the Treacher Collins syndrome (TCOF1) gene product. Gene 359, 73-80 (2005)

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