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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | X-11 + X-19 |
| Immunogen | nuclear pore complex-lamina fraction of XLKE-A6 cells (cultured cell line derived from Xenopus laevis) |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 100 (IHC, 50 µl/slide) |
| Intended use | Research use only |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | ready-to-use |
| Immunohistochemistry (IHC) - paraffin | ready-to-use (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
The mabs represent marker for nucleolar organizer region (NOR), putatively staining the protein treacle (described as TCOF1).
Polypeptides reacting: Mr 240 kD band in SDS gels and lower molecular weight bands (e.g. 160 kD band, Gonzales et al. 2005).
Gonzales B, Yang H, Henning D, Valdez BC: Cloning and functional characterization of the Xenopus orthologue of the Treacher Collins syndrome (TCOF1) gene product. Gene 359, 73-80 (2005)
Reference
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