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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | 23-5-14 |
| Immunogen | Nuclear fraction prepared from Xenopus laevis oocytes |
| Purification | hybridoma cell culture supernatant concentrate |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 2500 (IHC, 50 µl/slide); ≤ 150 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | xenopus (negative with other higher vertebrates) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:300 - 1:500 (fixation with paraformaldehyde recommended) |
| Immunoprecipitation (IP) | assay dependent |
| Western Blot (WB) | 1:3,000 |
Background
Details
The antibody recognizes a 125 kD nucleoplasmic protein (AND-1, acidic nucleoplasmic DNA-binding protein) in Xenopus.
Immunolocalization studies showed a dispersed, finely granular distribution pattern throughout the nucleoplasm.
Reference
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