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anti-nup153 mouse monoclonal, nup7A8, supernatant

The antibody recognizes the nuclear pore complex (NPC) protein nup153 (190 kD); also reactive with the soluble form of nup153.
Cat. No.: 651141
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone nup7A8
Immunogen nuclear matrix protein fraction prepared from rat liver nuclei
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage store at 2 – 8 °C
Tested species reactivity amphibia, human, marsupials, rodents

Applications

Immunohistochemistry (IHC) - frozen 1:5 – 1:10
Immunohistochemistry (IHC) - paraffin 1:5 – 1:10 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) 1:30 - 1:50

Background

Details

The antibody recognizes the nuclear pore complex (NPC) protein nup153 (190 kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e.g. during meiotic metaphase. Epitope in rat liver was mapped to N-terminal domain (aa439-611; cf. Hase & Cordes, 2003).

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