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anti-nup153 mouse monoclonal, nup7A8, supernatant

The antibody recognizes the nuclear pore complex (NPC) protein nup153 (190 kD); also reactive with the soluble form of nup153.
Cat. No.: 651141
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone nup7A8
Immunogen nuclear matrix protein fraction prepared from rat liver nuclei
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse, rat, rat kangoroo, xenopus

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5 – 1:10
Immunoprecipitation (IP) assay dependent
Western Blot (WB) 1:30 - 1:50

Background

Details

The antibody recognizes the nuclear pore complex (NPC) protein nup153 (190 kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e.g. during meiotic metaphase. Epitope in rat liver was mapped to N-terminal domain (aa439-611; Hase & Cordes, 2003).

Hase ME, Cordes VC: Direct interaction with nup153 mediates binding of tpr to the periphery of the nuclear pore complex. Mol Biol Cell 14, 1923-1940 (2003).

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