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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgM |
| Clone | mESL |
| Immunogen | synthetic C-terminus (21 aa) of human organic anion transporter SLC21A6 coupled to KLH |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 100 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | ready-to-use |
| Immunohistochemistry (IHC) - paraffin | ready-to-use (microwave treatment recommended) |
| Immunoprecipitation (IP) | assay dependent |
| Western Blot (WB) | assay dependent (for optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended; alternatively, samples should be kept at 37°C for 30 min) |
Background
Details
In Western blot analyses the monoclonal antibody mESL reacts selectively with the 90 kD protein (after SDS-PAGE) present in the basolateral membrane fractions of human liver. According to a recent new nomenclature this hepatocyte-specific protein is identical to OATP1B1 (Hagenbuch and Meier, 2003) also known as OATP-C.
Using immunofluorescence microscopy mESL stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma and the human cell line HEK293 transfected with SLC21A6.
Polypeptide reacting: OATP2/OATP1B1, human organic anion transporter SLC21A6 (90 kD).
Reactivity on cultured cell lines: SCL21A6 transfected HEK293 cells; negative with HepG2 and PLC.
Hagenbuch E, Meier PJ: The superfamily of organic anion transporting polypeptides. Biochim Biophys Acta 1609, 1-18 (2003)
Reference
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