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anti-Organic Anion Transporter OATP2 + P8 mouse monoclonal, mMDQ, supernatant

The anti-Organic Anion Transporter OATP2 + P8 antibody to human hepatic organic anion transporters SLC21A6 and SLC21A8 (OATP2/OATP8, also known as OATP1B1 or OATP-C and OATP1B3) detects the antigens upregulated in hepatocellular carcinoma.

Cat. No.: 651140
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone mMDQ
Immunogen synthetic N-terminus (24 aa) of human organic anion transporter OATP2
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent (for optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended; alternatively, samples can be kept at 37°C for 30 min)

Background

Details

In Western blot analyses of basolateral membrane fractions of human liver the monoclonal antibody mMDQ reacts with 90 kD and 120 kD proteins (after SDS-PAGE) representing OATP2 (also described as OATP-C) and OATP8, respectively. According to a recent new nomenclature these hepatocyte-specific proteins are identical to OATP1B1 and OATP1B3, respectively (Hagenbuch et al., 2003). Using immunofluorescence microscopy mMDQ stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma (Cui et al., 2003).

Tested reactivities on cultured sell lines: Transfected HEK293 cells; negative with HepG2 and PLC
Polypeptide reacting: OATP2 and OATP8, human organic anion transporters (90 kD, 120 kD, after SDS-PAGE)


Hagenbuch, B. & Meier, P. J. The superfamily of organic anion transporting polypeptides. Biochim. Biophys. Acta 1609, 1–18 (2003).
Cui, Y. et al. Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma. Lab. Invest. 83, 527–38 (2003).

Reference

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