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anti-p0071 guinea pig polyclonal, serum

The anti-p0071 antibody stains an armadillo plaque protein that characterizes a specific subtype of adherens junctions and their functions. This plaque protein (p0071) is specific for a subtype of adherens junctions, with a molecular mass of 135 kD.
Cat. No.: GP71
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€266.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide cocktail of human p0071: Peptide 1-1 to 1-4 (aa positions: aa 85-104; aa 173-193; aa 359-382; aa 407-430)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)
Immuno Electron Microscopy (IEM) assay dependent
Western Blot (WB) 1:1,000

Background

Details

Stains an armadillo plaque protein that characterizes a specific subtype of adherens junctions.
This plaque protein (p0071) is specific for a subtype of adherens junctions, with a molecular mass of 135 kD. p0071 is specifically detected in the plaques of the composite junctions (area compositae) of cardiac intercalated disks, in puncta adhaerentia of single- and multi-layered epithelia and blood vessel endothelial cells.; it is, however, absent from desmosomes.

Reactivity on cultured cell lines: MCF-7, MH1C1, 3T3, RVF-SMV

Positive control: MCF-7

Reference

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