anti-p0071 guinea pig polyclonal, serum

Cat. No. GP71

Available, delivery time 1-7 days

€274.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IEM, IHC and WB
  • Reacts with bovine, human and mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IEM, IHC, WB
Purification Stabilized antiserum
Reactivity Bovine, Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide cocktail of human p0071: Peptide 1-1 to 1-4 (aa positions: aa 85-104; aa 173-193; aa 359-382; aa 407-430)
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID Q99569 (Human)
Synonym Plakophilin-4, p0071, PKP4
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100
Immunohistochemistry (IHC) - frozen Assay dependent
Immunohistochemistry (IHC) - paraffin Assay dependent (microwave treatment recommended)
Immuno Electron Microscopy (IEM) Assay dependent
Western Blot (WB) 1:1,000
Background

Stains an armadillo plaque protein that characterizes a specific subtype of adherens junctions.
This plaque protein (p0071) is specific for a subtype of adherens junctions, with a molecular mass of 135 kD. p0071 is specifically detected in the plaques of the composite junctions (area compositae) of cardiac intercalated disks, in puncta adhaerentia of single- and multi-layered epithelia and blood vessel endothelial cells.; it is, however, absent from desmosomes.

Reactivity on cultured cell lines: MCF-7, MH1C1, 3T3, RVF-SMV

Positive control: MCF-7

References/Publications (3)
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