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anti-p0071 mouse monoclonal, 433.10.3, supernatant

The anti-p0071 antibody detects a plaque protein (p0071) specific for a subtype of adherens junctions, with a molecular mass of 135 kD.
Cat. No.: 651164
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 433.10.3
Immunogen Synthetic peptide of human p0071 (aa position 1170-1192: DFYSTKRPSYRAEQYPGSPDSWV)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) ready-to-use
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) 1:10 - 1:50

Background

Details

Detects a plaque protein (p0071) specific for a subtype of adherens junctions, with a molecular mass of 135 kD and is closely related to neurojungin / delta-catenin and ARVC (all belonging to the p120 armadillo subfamily). p0071 is specifically detected in the plaques of the composite junctions (area compositae) of cardiac intercalated disks, in puncta adhaerentia of single- and multi-layered epithelia and blood vessel endothelial cells.; it is, however, absent from desmosomes.
This protein was also described as plakophilin 4.
Reactivity on cultured cell lines: MCF-7, A431, CaCo-2, HT-29, PLC, 16HBE14o, HaCaT, SV80, U333 CG, 3T3, RVF-SMC, MH1C1
Positive control: MCF-7 cells

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