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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | 406.3.1 + 433.10.3 + 7.7.9 |
| Immunogen | synthetic peptides of human p0071 |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 100 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume) |
| Intended use | Research use only |
| Tested species reactivity | bovine, human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | ready-to-use |
| Immunohistochemistry (IHC) - frozen | ready-to-use |
| Immunohistochemistry (IHC) - paraffin | ready-to-use (microwave treatment recommended) |
| Western Blot (WB) | 1:10 – 1:50 |
Background
Details
Detects a plaque protein (p0071) specific for a subtype of adherens junctions, with a molecular mass of 135 kD and is closely related to neurojungin / delta-catenin and ARVC (all belonging to the p120 armadillo subfamily). p0071 is specifically detected in the plaques of the composite junctions (area compositae) of cardiac intercalated disks, in puncta adhaerentia of single- and multi-layered epithelia and blood vessel endothelial cells.; it is, however, absent from desmosomes. This protein was also described as plakophilin 4.
Reactivity on cultured cell lines: MCF-7, A431, CaCo-2, HT-29, PLC, 16HBE14o, HaCaT, SV80, U333 CG, 3T3, RVF-SMC, MH1C1.
Positive control: MCF-7 cells
Reference
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Species
Application
human
IHC-IF (frozen)
human
IHC-IF (frozen)
