PROGEN - passion for research

close×

anti-p21 Protein mouse monoclonal, WA-1, supernatant

p21 immunostaining is most often used in combination with p53 to confirm the presence of a p53 mutation. p53 overexpression in combination with low or absent p21 expression is consistent with a p53 mutation.
Cat. No.: 16081
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€260.00
Excl. 19% Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone WA-1
Immunogen Rec. WAF-1/p21 protein
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (most mammals)
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

p21 is a nuclear 21 kD protein, a product of the WAF-1/CIP1 gene. It is a constituent of a large complex of nuclear proteins, including cyclins, cyclin dependent kinases and PCNA. After activation by the wild type tumor suppressor protein p53, WAF-1 acts as an inhibitor of the cell cycle during G1 phase. Normal cells generally display a rather intense nuclear p21 expression. In several malignancies (e.g. breast cancer, bladder cancer, head and neck scc) it has been shown that the presence of mutations in the p53 gene are of prognostic significance. Demonstration of low expression of p21 in a p53 positive tumor will increase the likelihood of the presence of a p53 mutation. p21 immunostaining is therefore most often used in combination with p53 to confirm the presence of a p53 mutation. Overexpression of p53 with normal intensity of p21 argues against a p53 mutation, while p53 overexpression in combination of low or absent p21 expression is consistent with a p53 mutation.

Positive control: Tonsil.

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields