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p53 staining in human squamous cell carcinoma (image courtesy of J.Heß, University Hospital Heidelberg) Zoom

p53 staining in human squamous cell carcinoma (image courtesy of J.Heß, University Hospital Heidelberg)

anti-p53 Protein mouse monoclonal, Bp53.11, lyophilized, purified

The anti-p53 antibody reacts with wild-type and mutant forms of human p53 antigen (Immunogen: transcription domain within the NH2-terminal region). Stains 60% of investigated carcinomas of e.g. breast, colon, pancreas.
Cat. No.: 61039
Quantity:  2 x 25 µg

Delivery Time: usually 1-7 working days

€212.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone Bp53.11
Immunogen Recombinant human p53 (transcription domain within the NH2 terminus)
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:500
Immunohistochemistry (IHC) - paraffin 1:500 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

Excellent marker for wild-type and mutant forms of human p53 antigen. Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31. The antibody stains positively approx. 60 % of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.
Reactivity on cultured cell lines: Hela, MCF-7

Reference

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