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anti-p53 Protein mouse monoclonal, Bp53.11, supernatant

The anti-p53 antibody reacts with wild-type and mutant forms of human p53 antigen (Immunogen: transcription domain within the NH2-terminal region). Stains 60% of investigated carcinomas of e.g. breast, colon, pancreas.
Cat. No.: 65139
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone Bp53.11
Immunogen Recombinant human p53 (transcription domain within the NH2 terminus)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human

Applications

ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

Excellent marker for wild-type and mutant forms of human p53 antigen. Epitope recognized by Bp53.11: 20SDLWKLLPENNV31. The antibody stains positively approx. 60 % of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell leukaemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.

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