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p53 staining in human squamous cell carcinoma (image courtesy of J.Heß, University Hospital Heidelberg) Zoom

p53 staining in human squamous cell carcinoma (image courtesy of J.Heß, University Hospital Heidelberg)

anti-p53 Protein mouse monoclonal, Bp53.11, supernatant

The anti-p53 antibody reacts with wild-type and mutant forms of human p53 antigen (Immunogen: transcription domain within the NH2-terminal region). Stains 60% of investigated carcinomas of e.g. breast, colon, pancreas.
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Cat. No.: 65139
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone Bp53.11
Immunogen recombinant human p53 (transcription domain within the NH2 terminus)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended; do not apply proteases)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

Excellent marker for wild-type and mutant forms of human p53 antigen. Epitope recognized by Bp53.11: 20SDLWKLLPENNV31.
The antibody stains positively approx. 60 % of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell leukaemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.

Reactivity on cultured cell lines: Hela, MCF-7.

Reference

Reference

PublicationSpeciesApplication
Ratnaparkhe, M. et al. Defective DNA damage repair leads to frequent catastrophic genomic events in murine and human tumors. Nat.Commun. 9, 4760 (2018). human WB
Kolb, T., Maass, K., Hergt, M., Aebi, U. & Herrmann, H. Lamin A and lamin C form homodimers and coexist in higher complex forms both in the nucleoplasmic fraction and in the lamina of cultured human cells. Nucleus 2, 425–33 (2011). human WB
Ohtaké, S. et al. Precancerous foci in pleomorphic adenoma of the salivary gland: recognition of focal carcinoma and atypical tumor cells by P53 immunohistochemistry. J. Oral Pathol. Med. 31, 590–7 (2002). human IHC (paraffin)
Homann, N. et al. Overexpression of p53 in tumor-distant epithelia of head and neck cancer patients is associated with an increased incidence of second primary carcinoma. Clin. Cancer Res. 7, 290–296 (2001). human IHC (frozen)
Vispo, N. S., Araña, M. J., Chinea, G., Ojalvo,  a G. & Cesareni, G. Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions. Hybridoma 18, 251–255 (1995). epitope mapping
Petersen, G., Song, D., Hugle-Dorr, B., Oldenburg, I. & Bautz, E. K. Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries. Mol Gen Genet 249, 425–431 (1995). epitope mapping
Bártek, J. et al. Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies. J. Pathol. 169, 27–34 (1993). human WB, IHC (paraffin)

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