anti-p62/ SQSTM1 (C-terminus) guinea pig polyclonal + anti-guinea pig IgG HRP set
|Quantity||100 µl anti-p62 + 100 µg anti-guinea pig IgG HRP|
|Host||Goat, Guinea pig|
|Reactivity||See individual antibody datasheet|
|Storage||Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)|
|Intended use||Research use only|
|Immunogen||See individual antibody datasheet for information about specific immunogens|
|Formulation||GP62-C antibody: contains 0.09% sodium azide; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µl dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||anti-p62: 1:100-1:600|
|Immunohistochemistry (IHC) - paraffin||anti-p62: 1:100-1:600 (microwave treatment recommended)|
|Western Blot (WB)||anti-p62: 1:1,000-1:3,000; anti-guinea pig HRP: 1:2,500-1:100,000|
The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy and for detection of neurofibrillary tangles in the brain of Alzheimer disease patients, in Parkinson diseases and various chronic liver diseases.
Human 62 kD (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy).
The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease.
anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
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- Validated and tested western blot control for: anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal antibody, Cat. No. GP62-C