anti-p62/ SQSTM1 (C-terminus) + positive western blot control
|Quantity||100 µl anti-p62 + 50 µg western blot control|
|Purification||Cell culture lysate, Stabilized antiserum|
|Reactivity||Bovine, Human, Mouse, Rat|
|Storage||Antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/thaw cycles for both components|
|Intended use||Research use only|
|Immunogen||C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat.|
|Formulation||antibody: contains 0.09% sodium azide; western blot control: lyophilized, reconstitute in 50 µl 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||anti-p62: 1:100-1:600|
|Immunohistochemistry (IHC) - paraffin||anti-p62: 1:100-1:600 (microwave treatment recommended)|
|Western Blot (WB)||anti-p62: 1:1,000-1:3,000; western blot control: 10 µg total protein per lane|
The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy and for detection of neurofibrillary tangles in the brain of Alzheimer disease patients, in Parkinson diseases and various chronic liver diseases.
Human 62 kDa (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy).
The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease.
The GP62-C antibody is knockout validated (Waguri & Komatsu, 2009).
Positive western blot control:
Whole Cell Lysate from PLC/PRF/5 human Hepatoma cell line.
PLC whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.
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- Whole cell lysate from PLC human hepatoma cell line (PLC/PRF/5)
- Validated and tested western blot control for: anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal antibody, Cat. No. GP62-C