Frequently used

anti-p62/ SQSTM1 (C-terminus) + positive western blot control

Cat. No. GP62-C-WBC

Available, delivery time 1-7 days

€329.00*

Key Features
  • Set provides anti-p62 antibody and associated western blot control - positively tested PLC cell lysate.
  • The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy.

Included products:

Product description
Quantity 100 µl anti-p62 + 50 µg western blot control
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Cell culture lysate, Stabilized antiserum
Reactivity Bovine, Human, Mouse, Rat
Storage Antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/thaw cycles for both components
Intended use Research use only
Immunogen C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat.
Formulation antibody: contains 0.09% sodium azide; western blot control: lyophilized, reconstitute in 50 µl 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-p62: 1:100-1:600
Immunohistochemistry (IHC) - paraffin anti-p62: 1:100-1:600 (microwave treatment recommended)
Western Blot (WB) anti-p62: 1:1,000-1:3,000; western blot control: 10 µg total protein per lane
Background

anti-p62 antibody:
The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy and for detection of neurofibrillary tangles in the brain of Alzheimer disease patients, in Parkinson diseases and various chronic liver diseases.
Human 62 kDa (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy).
The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease.

The GP62-C antibody is knockout validated (Waguri & Komatsu, 2009).

Waguri, S. & Komatsu, M. Chapter 9 Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice. Methods in Enzymology 453, 181–196 (2009).

Positive western blot control:
Whole Cell Lysate from PLC/PRF/5 human Hepatoma cell line.
PLC whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.


References/Publications (208)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 917.17 KB
Filetype pdf
Category Protocol
Size 919.99 KB
Filetype pdf
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


Related Products

Positive western blot control: anti-p62/SQSTM1 antibody
Cat. No. 64006
  • Whole cell lysate from PLC human hepatoma cell line (PLC/PRF/5)
  • Validated and tested western blot control for: anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal antibody, Cat. No. GP62-C

€70.00*
anti-p62/ SQSTM1 (C-terminus) guinea pig polyclonal, serum
Cat. No. GP62-C
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, mouse and rat

€299.00*