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Human PLC cells

anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal, serum

The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy and for detection of neurofibrillary tangles in the brain of Alzheimer disease patients, in Parkinson diseases and various chronic liver diseases. Human p62 protein, present in intracytoplasmic inclusions is found in many tissues and cells, serving probably a common cellular signal transduction mechanism.
Cat. No.: GP62-C
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€279.00
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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat.
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 – 1:200
Immunohistochemistry (IHC) - paraffin 1:100 – 1:200 (microwave treatment recommended)
Western Blot (WB) 1:1000 – 1:2000

Background

Details

Human 62 kD (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy).
The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer’s disease.

Reference

Reference

PublicationSpeciesApplication
Santanam, U. et al. Atg7 cooperates with Pten loss to drive prostate cancer tumor growth. Genes Dev. 30, 399–407 (2016). mouse WB
Sergin, I. et al. p62-enriched inclusion bodies in macrophages protect against atherosclerosis HHS Public Access. Sci. Signal. 9, (2016). mouse IHC (frozen), ICC-IF
Starheim, K. et al. Intracellular glutathione determines borte. cytotoxicity in multiple myeloma cells. Blood Cancer J. 6, (2016). human WB
Yamanaka, T. et al. Differential roles of NF-Y transcription factor in ER chaperone expression and neuronal maintenance in the CNS. Nat. Publ. Gr. 6, (2016). mouse WB, IHC (frozen)
Lim, S. W., Jin, L., Jin, J. & Yang, C. W. Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus. Nat. Publ. Gr. 6, (2016). rat WB, IHC (paraffin)
Angelini, C., Nascimbeni, A. C., Cenacchi, G. & Tasca, E. Lipolysis and lipophagy in lipid storage myopathies. Biochim. Biophys. Acta 1367–1373 (2016). human WB, IHC
Corcelle-Termeau, E. et al. Excess sphingomyelin disturbs ATG9A trafficking and autophagosome closure. Autophagy 12, 533–849 (2016) mouse WB
Ra, E. A. et al. TRIM31 promotes Atg5/Atg7-independent autophagy in intestinal cells. Nat. Commun. 7, (2016). human WB
Kaizuka, T. & Mizushima, N. Atg13 Is Essential for Autophagy and Cardiac Development in Mice. Mol. Cell. Biol. 36, 585–595 (2016). mouse WB, ICC-IF
Saito, T. et al. p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming. Nat. Commun. 7, (2016). human, mouse WB, IHC (paraffin)
Jarvela, T. S. et al. The neural chaperone proSAAS blocks α-synuclein fibrillation and neurotoxicity. Proc. Natl. Acad. Sci. U. S. A. (2016). human IHC (frozen)
Puvirajesinghe, T. M. et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2–JNK signalling in breast cancer. Nat. Commun. 7, (2016). human, mouse WB, ICC-IF
Salas, E., Roy, S., Marsh, T., Rubin, B. & Debnath, J. Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death. Oncogene 35, 2913–2922 (2016). mouse ICC-IF
Klionsky, D. J. et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12, 1 (2016). mouse WB
Zhang, J. et al. A Founder Mutation in VPS11 Causes an Autosomal Recessive Leukoencephalopathy Linked to Autophagic Defects. PLoS Genet. 12,(2016). human WB
Kenific, C. M. et al. NBR1 enables autophagy-dependent focal adhesion turnover. J. Cell Biol. 212, 577–90 (2016). human WB
Taneike, M. et al. mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy. PLoS One 11, (2016). mouse WB
Morimoto, D. et al. The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates. Nat. Commun. 6, (2015). mouse WB, ICC-IF
Echeverry, N. et al. Inhibition of autophagy sensitizes malignant pleural mesothelioma cells to dual PI3K/mTOR inhibitors. Cell Death Dis. 6, (2015). human WB
Milan, E. et al. A plastic SQSTM1/p62-dependent autophagic reserve maintains proteostasis and determines proteasome inhibitor susceptibility in multiple myeloma cells. Autophagy 11, 1161–1178 (2015). human WB, ICC-IF
Lenoir, O. et al. Endothelial cell and podocyte autophagy synergistically protect from diabetes-induced glomerulosclerosis. Autophagy 11, 1130–45 (2015). mouse WB
Larroquette, F. et al. Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response. Hum. Mol. Genet. 24, 6515–6529 (2015). mouse WB
Hieke, N. et al. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy 11, 1471–1483 (2015). mouse WB, ICC-IF
Zhou, Z. et al. Autophagy supports color vision. Autophagy 11, 1821–1832 (2015). mouse IHC (frozen)
Ciuffa, R. et al. The selective autophagy receptor p62 forms a flexible filamentous helical scaffold. Cell Rep. 11, 748–58 (2015). human WB
Ulbricht, A. et al. Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle. Autophagy 11, 538–546 (2015). human WB
Murrow, L., Malhotra, R. & Debnath, J. ATG12-ATG3 Interacts with Alix to Promote Basal Autophagic Flux and Late Endosome Function. Nat. Cell Biol. 17, 300–310 (2015). mouse WB
Lim, J. et al. Proteotoxic Stress Induces Phosphorylation of p62/SQSTM1 by ULK1 to Regulate Selective Autophagic Clearance of Protein Aggregates. PLoS One 11, (2015). human, mouse WB, ICC-IF
Pellegrini, C. et al. All-trans retinoic acid and rapamycin normalize Hutchinson Gilford progeria fibroblast phenotype. Oncotarget 6, 29914–29928 (2015). human WB
Eino, A. et al. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. J. Cell Sci. 128, 4453–61 (2015). mouse IHC (paraffin)
Felzen, V. et al. Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function. Cell Death Dis. 6, (2015). human ICC-IF
Chandrachud, U. et al. Unbiased Cell-based Screening in a Neuronal Cell Model of Batten Disease Highlights an Interaction between Ca 2+ Homeostasis, Autophagy, and CLN3 Protein Function. J. Biol. Chem. 290, 14361–14380 (2015). mouse WB
Liu, S. et al. Disrupted autophagy after spinal cord injury is associated with ER stress and neuronal cell death. Cell Death Dis. 6, (2015). rat WB, IHC (frozen)
McMillan, E. M. et al. Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. PLoS One 10, e0119382 (2015). rat WB
Johansson, I. et al. The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells. Autophagy 11, 1636–1651 (2015). human, mouse WB, ICC-IF
Lodder, J. et al. Macrophage autophagy protects against liver fibrosis in mice. Autophagy 11, 1280–1292 (2015). mouse ICC-IF
Agarwal, S. et al. Autophagy and endosomal trafficking inhibition by Vibrio cholerae MARTX toxin phosphatidylinositol- 3-phosphate-specific phospholipase A1 activity. Nat. Commun. 6, (2015). human WB
Akizu, N. et al. Biallelic mutations in SNX14 cause a syndromic form of cerebellar atrophy and lysosome-autophagosome dysfunction HHS Public Access. Nat. Genet. 47, 528–534 (2015). human WB
Ruozi, G. et al. AAV-mediated in vivo functional selection of tissue-protective factors against ischaemia. Nat. Commun. 6, (2015). mouse WB
Arel-Dubeau, A.-M. et al. Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons. Oxid. Med. Cell. Longev. 2014, 15 (2015). rat WB
Clayton, E. L. et al. Frontotemporal dementia caused by CHMP2B mutation is characterised by neuronal lysosomal storage pathology. Acta Neuropathol. 130, 511–23 (2015). mouse IHC (paraffin), IEM
Newton, P. T., Vuppalapati, K. K., Bouderlique, T. & Chagin, A. S. Pharmacological inhibition of lysosomes activates the MTORC1 signaling pathway in chondrocytes in an autophagy-independent manner. Autophagy 11, 1594–1607 (2015). mouse, rat WB
Shi, J. et al. NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. Cell Death Dis. 6, (2015). human WB
Khan, M. M. et al. Role of autophagy, SQSTM1, SH3GLB1, and TRIM63 in the turnover of nicotinic acetylcholine receptors. Autophagy 10, 123–36 (2014). mouse WB, IHC
Costes, S., Gurlo, T., Rivera, J. F. & Butler, P. C. UCHL1 deficiency exacerbates human islet amyloid polypeptide toxicity in β-cells: evidence of interplay between the ubiquitin/proteasome system and autophagy. Autophagy 10, 1004–14 (2014). mouse WB, IHC (paraffin)
Karlsson, A. B. et al. The role of spartin and its novel ubiquitin binding region in DALIS occurrence. Mol. Biol. Cell 25, 1355–1365 (2014). mouse ICC-IF
Dunlop, E. A. et al. FLCN, a novel autophagy component, interacts with GABARAP and is regulated by ULK1 phosphorylation. Autophagy 10, 1749–1760 (2014). human, mouse WB, IHC
Nicot, A.-S. et al. Phosphorylation of NBR1 by GSK3 modulates protein aggregation. Autophagy 10, 1036–53 (2014). human WB
Rivera, J. F., Costes, S., Gurlo, T., Glabe, C. G. & Butler, P. C. Autophagy defends pancreatic β cells from human islet amyloid polypeptide-induced toxicity. J. Clin. Invest. 124, 3489–500 (2014). mouse WB, IHC (paraffin)
Kinsey, C. et al. Plac8 links oncogenic mutations to regulation of autophagy and is critical to pancreatic cancer progression. Cell Rep. 7, 1143–1155 (2014). human, mouse WB
Sahani, M. H., Itakura, E. & Mizushima, N. Expression of the autophagy substrate SQSTM1/p62 is restored during prolonged starvation depending on transcriptional upregulation and autophagy-derived amino acids. Autophagy 10, 431–41 (2014). mouse WB, ICC-IF
Guan, B.-J. et al. Translational Control during Endoplasmic Reticulum Stress beyond Phosphorylation of the Translation Initiation Factor eIF2␣ *. J. Biol. Chem. 289, 12593–12611 (2014). mouse WB
Lim, Y.-M. et al. Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes. Nat. Commun. 5, (2014). mouse WB
Ricketts, T. et al. A nonsense mutation in mouse Tardbp affects TDP43 alternative splicing activity and causes limb-clasping and body tone defects. PLoS One 9, (2014). mouse IHC (paraffin)
Kondratskyi, A. et al. Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death. Cell Death Dis. 5, (2014). human WB
Tanaka, K. et al. A Novel Acylaminoimidazole Derivative, WN1316, Alleviates Disease Progression via Suppression of Glial Inflammation in ALS Mouse Model. PLoS One 9, (2014). mouse WB
Heijnen, H. F. et al. Ribosomal Protein Mutations Induce Autophagy through S6 Kinase Inhibition of the Insulin Pathway. PLoS Genet. 10, (2014). human WB, ICC-IF
Ost, M., Werner, F., Dokas, J., Klaus, S. & Voigt, A. Activation of AMPKa2 Is Not Crucial for Mitochondrial Uncoupling-Induced Metabolic Effects but Required to Maintain Skeletal Muscle Integrity. PLoS One 9, (2014). mouse WB
Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014). mouse IHC (frozen)
Sarkar, C. et al. Impaired autophagy flux is associated with neuronal cell death after traumatic brain injury. Autophagy 10, 2208–2222 (2014). mouse IHC (frozen)
Cottam, E. M., Whelband, M. C. & Wileman, T. Coronavirus NSP6 restricts autophagosome expansion. Autophagy 10, (2014). monkey WB, ICC-IF
Gabandé-Rodrıuez, E., Boya, P., Labrador, V., Dotti, C. & Ledesma, M. High sphingomyelin levels induce lysosomal damage and autophagy dysfunction in Niemann Pick disease type A. Cell Death Differ. 21, 864–875 (2014). mouse WB
Xu, X. et al. Autophagy is essential for effector CD8 T cell survival and memory formation. Nat. Immunol. 15, 1152–1161 (2014). mouse WB
Furuya, N. et al. PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. Autophagy 10, 631–41 (2014). mouse WB
Zhang, L. et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat. Commun. 5, (2014). mouse WB, IHC (paraffin), ICC-IF, IP
Yamanaka, T. et al. NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat. Commun. 5, (2014). mouse WB, IHC (frozen), IEM
Kageyama, S. et al. Proteasome Dysfunction Activates Autophagy and the Keap1-Nrf2 Pathway. J. Biol. Chem. 289, 24944–24955 (2014). mouse WB, IHC (frozen)
Yamano, K. & Youle, R. J. PINK1 is degraded through the N-end rule pathway. Autophagy 9, 1758–69 (2013). human ICC-IF
Kondylis, V. et al. Endosome-mediated autophagy: an unconventional MIIC-driven autophagic pathway operational in dendritic cells. Autophagy 9, 861–80 (2013). mouse WB, ICC-IF, IEM
Yuan, H.-X., Russell, R. C. & Guan, K.-L. Regulation of PIK3C3/VPS34 complexes by MTOR in nutrient stress-induced autophagy. Autophagy 9, 1983–95 (2013). human, mouse WB
Jiang, S., Guo, R., Zhang, Y., Zou, Y. & Ren, J. Heavy metal scavenger metallothionein mitigates deep hypothermia-induced myocardial contractile anomalies: role of autophagy. Am. J. Physiol. - Endocrinol. Metab. 304, (2013). mouse WB
Shackelford, D. B. et al. LKB1 inactivation dictates therapeutic response of non-small cell lung cancer to the metabolism drug phenformin. Cancer Cell 23, 143–58 (2013). human WB, IHC
Raina, K., Agarwal, C., Wadhwa, R., Serkova, N. J. & Agarwal, R. Energy deprivation by silibinin in colorectal cancer cells: a double-edged sword targeting both apoptotic and autophagic machineries. Autophagy 9, 697–713 (2013). mouse WB, IHC
Schober, A. et al. Cell Loss and Autophagy in the Extra-Adrenal Chromaffin Organ of Zuckerkandl are Regulated by Glucocorticoid Signalling. J. Neuroendocrinol. 25, 34–47 (2013). mouse IHC (frozen)
Adolph, T. E. et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272–276 (2013). mouse WB
Li-Hsuan Huang, M. et al. Molecular and Functional Alterations in a Mouse Cardiac Model of Friedreich Ataxia Activation of the Integrated Stress Response, eIF2a Phosphorylation, and the Induction of Downstream Targets. Am. J. Pathol. 183, 745–757 (2013). mouse WB
Ozcelik, S. et al. Rapamycin Attenuates the Progression of Tau Pathology in P301S Tau Transgenic Mice. PLoS One 8, (2013). mouse WB
Warr, M. R. et al. FoxO3a Directs a Protective Autophagy Program in Hematopoietic Stem Cells. Nature 494, 323–327 (2013). mouse FACS
Bechtel, W. et al. Vps34 Deficiency Reveals the Importance of Endocytosis for Podocyte Homeostasis. J. Am. Soc. Nephrol. 24, 727–743 (2013). mouse WB, IHC (paraffin), ICC-IF
Ichimura, Y. et al. Phosphorylation of p62 Activates the Keap1-Nrf2 Pathway during Selective Autophagy. Mol. Cell 51, 618–631 (2013). human, mouse WB, ICC-IF
Hoshino, A. et al. Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart. Nat. Commun. 4, 9971–9981 (2013). mouse WB
Ghazi-Noori, S. et al. Progressive neuronal inclusion formation and axonal degeneration in CHMP2B mutant transgenic mice. Brain 135, 819–832 (2012). mouse IHC (paraffin)
Liu, S. et al. Autophagy plays a critical role in kidney tubule maintenance, aging and ischemia-reperfusion injury. Autophagy 8, 826–837 (2012). mouse WB, IHC (frozen, paraffin)
Stingele, S. et al. Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cells. Mol. Syst. Biol. 8, (2012). human WB, ICC-IF

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