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anti-p62 / SQSTM1 (N-terminus) guinea pig polyclonal, serum

Human p62 protein, present in intracytoplasmic inclusions is found in many tissues and cells, serving probably a common cellular signal transduction mechanism.
Cat. No.: GP62-N
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€266.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen N-terminal domain (20 amino acids: MAS LTV KAY LLG KED AAR EI-C) of human p62 protein, coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID Q13501
Tested species reactivity human, rat (negative with mouse)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) 1:1,000

Background

Details

Reactive polypeptide (Specificity): Human 62 kD (p62) protein, present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. p62 protein has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism. The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer’s disease.

Reference

Reference

PublicationSpeciesApplication
Yang, S. et al. Defective mitophagy driven by dysregulation of rheb and KIF5B contributes to mitochondrial reactive oxygen species (ROS)-induced nod-like receptor 3 (NLRP3) dependent proinflammatory response and aggravates lipotoxicity. Redox Biol. 3, 63–71 (2014). rat WB
Roberts, R. et al. Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery. Autophagy 9, 667–82 (2013). mouse, hamster ICC-IF
Li, S. et al. Cathepsin B Contributes to Autophagy-related 7 (Atg7)-induced Nod-like Receptor 3 (NLRP3)-dependent Proinflammatory Response and Aggravates Lipotoxicity in Rat Insulinoma Cell Line. J. Biol. Chem. 288, 30094–30104 (2013). rat WB
Kley, R. A. et al. Pathophysiology of protein aggregation and extended phenotyping in filaminopathy. Brain a J. Neurol. 135, 2642–60 (2012). human IHC (frozen)
Raben, N. et al. Suppression of autophagy permits successful enzyme replacement therapy in a lysosomal storage disorder--murine Pompe disease. Autophagy 6, 1078–89 (2010). mouse WB
Kuusisto, E., Kauppinen, T. & Alafuzoff, I. Use of p62/SQSTM1 antibodies for neuropathological diagnosis. Neuropathol. Appl. Neurobiol. 34, 169–180 (2008). human IHC (paraffin)
Furukawa, Y. et al. Ubiquitin and ubiquitin-related proteins in neurons and dendrites of brains of atypical Pick’s disease without Pick bodies. Neuropathol. Off. J. Japanese Soc. Neuropathol. 24, 38–45 (2004). human IHC (paraffin)
Arai, T. et al. Neuronal and glial inclusions in frontotemporal dementia with or without motor neuron disease are immunopositive for p62. Neurosci. Lett. 342, 41–4 (2003). human IHC (frozen)
Kuusisto, E., Salminen, A. & Alafuzoff, I. Early accumulation of p62 in neurofibrillary tangles in Alzheimer’s disease: possible role in tangle formation. Neuropathol. Appl. Neurobiol. 28, 228–37 (2002). human IHC (paraffin)
Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255–262 (2002). human, mouse WB, IHC (frozen, paraffin), ICC-IF

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