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anti-p97 ATPase mouse monoclonal, 58.13.3, lyophilized, purified

p97 ATPase (VCP) is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p. The antibody reacts with p97 subunit of 15S Mg2+-ATPase.
Cat. No.: 61078
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€195.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 58.13.3
Immunogen 15S Mg2+ ATPase complex purified from Xenopus laevis ovary
Purification hybridoma cell culture supernatant concentrate
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, rabbit, rat, xenopus (not with budding yeast Cdc48p)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) (not recommended)
Immunoprecipitation (IP) (not recommended)
Western Blot (WB) assay dependent

Background

Details

p97 ATPase / VCP (also described as valosin-containing protein) is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p.
The antibody reacts with the p97 subunit of 15S Mg2+-ATPase. p97/VCP is involved in ubiquitin-proteasome dependent protein degradation processes and involvement was also found e.g. in the formation of neuronal inclusion bodies in neuro-degenerative diseseases (e.g. Parkinson’s disease).

Positive control: Immunoblots with protein extracts from all vertebrate tissues and cells.

Reactivity on cultured cell lines: All vertebrate cells and tissues tested so far: e.g. Xenopus laevis; rabbit, rat, human; does not crossreact with the corresponding protein in budding yeast (Cdc48p)

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