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WB with anti-p97 ATPase antibody (Cat. No. 65278, 1:2000), 3T3 whole cell lysate (5-10 µg) Zoom

WB with anti-p97 ATPase antibody (Cat. No. 65278, 1:2000), 3T3 whole cell lysate (5-10 µg)

anti-p97 ATPase mouse monoclonal, 58.13.3, supernatant concentrate

p97 ATPase (VCP) s implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p. The antibody reacts with p97 subunit of 15S Mg2+-ATPase.
Cat. No.: 65278
Quantity:  500 µL

Delivery Time: usually 1-7 working days

€206.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 58.13.3
Immunogen 15S Mg2+ ATPase complex purified from Xenopus laevis ovary
Purification hybridoma cell culture supernatant concentrate
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, rabbit, rat, xenopus (not with budding yeast Cdc48p)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) not recommended
Immunoprecipitation (IP) not recommended
Western Blot (WB) 1:2,000 – 1:5,000

Background

Details

p97 ATPase / VCP (also described as valosin-containing protein) is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p.
The antibody reacts with the p97 subunit of 15S Mg2+-ATPase. p97/VCP is involved in ubiquitin-proteasome dependent protein degradation processes and involvement was also found e.g. in the formation of neuronal inclusion bodies in neuro-degenerative diseseases (e.g. Parkinson’s disease).
Positive control: Immunoblots with protein extracts from all vertebrate tissues and cells

Reference

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