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anti-p97 ATPase mouse monoclonal, 58.13.3, supernatant concentrate

p97 ATPase (VCP) s implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p. The antibody reacts with p97 subunit of 15S Mg2+-ATPase.
Cat. No.: 65278
Quantity:  500 µL

Delivery Time: usually 1-7 working days

€206.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 58.13.3
Immunogen 15S Mg2+ ATPase complex purified from Xenopus laevis ovary
Purification hybridoma cell culture supernatant concentrate
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, rabbit, rat, xenopus (not with budding yeast Cdc48p)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) not recommended
Immunoprecipitation (IP) not recommended
Western Blot (WB) 1:1,000 – 1:2,000

Background

Details

p97 ATPase / VCP (also described as valosin-containing protein) is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p.
The antibody reacts with the p97 subunit of 15S Mg2+-ATPase. p97/VCP is involved in ubiquitin-proteasome dependent protein degradation processes and involvement was also found e.g. in the formation of neuronal inclusion bodies in neuro-degenerative diseseases (e.g. Parkinson’s disease).
Positive control: Immunoblots with protein extracts from all vertebrate tissues and cells

Reference

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