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anti-PACAP-Related Peptide rabbit polyclonal, serum

The 29 aa pituitary adenylate cyclase activating polypeptide-related peptide is produced with PACAP-38 and PACAP-27 during procession of preproPACAP. It is abundant in brain, but is also found in the gastrointestinal tract.
Cat. No.: 16064
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€380.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host rabbit
Antibody Type polyclonal
Immunogen Synthetic rat PRP (Pensinsula), conjugated to BSA
Purification undiluted antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 100 µl dist. water
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity rat (negative with human)
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:400 - 1:800

Background

Details

Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. PACAP-related peptide (PRP) is a 29-amino acid peptide, which is produced together with PACAP-27 and PACAP-38 when PreproPACAP is being processed. PRP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts.
Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while PACAP-27, PACAP-38, VIP, PHI, CRF, oxytocin and vasopressin do not.
Positive control: Stefanini-fixed frozen sections of rat duodenum, fundus or antrum.

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