anti-pan AAV (intact particle) human recombinant, 1C4H

A) Dot blot analysis showing the comparison of cross-reactivity of the anti-pan AAV (human recombinant antibody, IgG1, cat. number 692373) with a multi-serotype recognizing AAV antibody from another vendor. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 1 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to verify binding of the antibody to conformational epitopes. Please note that the two antibody experiments were carried out on separate occasions with different secondary antibodies, which could impact the reliability of direct dot blot comparison.

B) Cross-reactivity of the anti-pan AAV (human recombinant antibody, IgG1, cat. number 692373) with AAV serotypes and variants, using dot blot analysis. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 0.2 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to exclude binding to non-assembled capsids.

Liquid

1 ml (50 µg/ml)

Liquid

200 µl (50 µg/ml)

Cat. No. 692373

Prices excl. VAT

Available, delivery time: 1-7 days

€345.00*

Datasheet

Key Features

Human recombinant antibody
Suitable for dot blot, ELISA and neutralization
Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAVmyo4A, AAVmyo4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9, AAVDJ, intact particles
Isotype: Human IgG1

Product description

Quantity	1 ml
Antibody Type	Recombinant
Host	human recombinant
Isotype	IgG1
Conjugate	Unconjugated
Application	Dot blot, ELISA, Neutralization assay
Purification	Affinity chromatography
Reactivity	AAV-F, AAV-LK03, AAV-PHP.eB, AAV1, AAV2, AAV2.7m8, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV9-P5, AAV11, AAV13, AAVAnc80L65, AAVDJ, AAVhu.37, AAVmyo, AAVmyo4A, AAVmyo4E, AAVrh10, AAVrh74, scAAV9
Storage	2-8°C
Intended use	Research use only
Clone	1C4H
Immunogen	AAV capsids
Concentration	50 µg/ml
Formulation	PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA

Applications

Tested applications	Tested dilutions
Dot Blot	1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA	Assay dependent
Neutralization Assay	EC50 ~ 47 ng/ml (AAV1), ~ 300 ng/ml (AAV2), ~ 121 ng/ml (AAV3), ~ 1627 ng/ml (AAV5), ~ 52 ng/ml (AAV6), ~ 72 ng/ml (AAV8), ~ 104 ng/ml (AAV9), ~ 543 ng/ml (AAVrh10), assay dependent

Background

The anti-pan AAV is a recombinant human antibody, specifically recognizing a conformational epitope only present on fully assembled and intact AAV capsids. It does not detect denatured AAV capsids, making it a versatile tool for various applications, such as tracking the localization of viral particles, studying protein-protein interactions, facilitating AAV particle purification, and determining total AAV capsid titers. Additionally, the antibody exhibits neutralizing activity against multiple AAV serotypes. The anti-pan AAV demonstrates broad binding capabilities across all AAV serotypes, with the exception of AAV12. Furthermore, it binds to numerous additional AAV variants, underscoring its versatility in diverse research and quality control settings.

_{Limited Use Label License: Research Use Only
Product is exclusively owned by PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.}

Downloads

Datasheet

MSDS

FAQs

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Contact

Need more product information?

Learn more

Related Products

AAV1 ELISA Control

Cat. No. PRAAV1-C

Consists of fully assembled, empty AAV1 capsids
Represents serotype-specific conformational epitopes
Reliable positive control for ELISA

€110.00*

AAV1 empty capsids

Cat. No. 66V010

Fully assembled, empty AAV1 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV1 standard material (eGFP)

Cat. No. 66V011

Fully assembled AAV1 capsids filled with an eGFP reporter gene controlled by a CMV promoter
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€580.00*

AAV1 Titration ELISA

Cat. No. PRAAV1

ELISA for quantitation of AAV1 capsids
Detection of full and empty AAV1 capsids
ADK1a antibody is used as capture and detection antibody

€560.00*

AAV2 ELISA Control

Cat. No. PRAAV2-C

Consists of fully assembled, empty AAV2 capsids
Represents serotype-specific conformational epitopes
Reliable positive control for ELISA

€110.00*

AAV2 empty capsids

Cat. No. 66V020

Fully assembled, empty AAV2 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV2 standard material (eGFP)

Cat. No. 66V021

Fully assembled AAV2 capsids filled with an eGFP reporter gene controlled by a CMV promoter
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
Quality controlled for purity, filling grade, aggregation, total capsid and genome titer

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€580.00*

AAV2 Titration ELISA

Cat. No. PRATV

ELISA for quantitation of AAV2 capsids
Detection of full and empty AAV2 capsids
A20 antibody is used as capture and detection antibody

€560.00*

AAV2 Titration ELISA 2.0R

Cat. No. PRAAV2R

ELISA for quantitation of AAV2 capsids
Detection of full and empty AAV2 capsids
A20R antibody is used as capture and detection antibody

€560.00*

AAV2 VP1 + VP2 + VP3, recombinant proteins, set

Cat. No. 72001

•    Recombinant AAV2 VP1-3 capsid protein set
•    Useful for ratio analysis
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.

For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€753.00*

AAV2 VP1, recombinant protein

Cat. No. 640823

•   Recombinant AAV2 capsid protein VP1
•   Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•   Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.

For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€291.00*

AAV2 VP2, recombinant protein

Cat. No. 640824

•    Recombinant AAV2 capsid protein VP2
•    Useful in combination with AAV2 VP1 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.

For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€291.00*

AAV2 VP3, recombinant protein

Cat. No. 640825

•    Recombinant AAV2 capsid protein VP3
•    Useful in combination with AAV2 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72001)
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.

For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€291.00*

AAV2 Xpress ELISA

Cat. No. PRAAV2XP

Fast, easy and reliable AAV2 titer determination:

get results in less than 2 hours and increase your daily sample throughput
easy workflow & process integration
same accuracy and reproducibility as the standard AAV2 Titration ELISAs
A20R antibody is used as capture and detection antibody

€685.00*

AAV3 ELISA Control

Cat. No. PRAAV3-C

Consists of fully assembled, empty AAV3 capsids
Represents serotype-specific conformational epitopes
Reliable positive control for ELISA

€110.00*

AAV3 empty capsids

Cat. No. 66V030

Fully assembled, empty AAV3 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV3 standard material (eGFP)

Cat. No. 66V031

Fully assembled AAV3 capsids filled with an eGFP reporter gene controlled by a CMV promoter
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€580.00*

AAV3 Titration ELISA 2.0R

Cat. No. PRAAV3R

ELISA for quantitation of AAV3 capsids
Detection of full and empty AAV3 capsids
A20R antibody is used as capture and detection antibody

€560.00*

AAV5 ELISA Control

Cat. No. PRAAV5-C

Consists of fully assembled, empty AAV5 capsids
Represents serotype-specific conformational epitopes
Reliable positive control for ELISA

€110.00*

AAV5 empty capsids

Cat. No. 66V050

Fully assembled, empty AAV5 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV5 standard material (eGFP)

Cat. No. 66V051

Fully assembled AAV5 capsids filled with an eGFP reporter gene controlled by a CMV promoter
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€580.00*

AAV5 Titration ELISA

Cat. No. PRAAV5

ELISA for quantitation of AAV5 capsids
Detection of full and empty AAV5 capsids
ADK5a antibody is used as capture and detection antibody

€560.00*

AAV5 VP1 + VP2 + VP3, recombinant proteins, set

Cat. No. 72005

•    Recombinant AAV5 VP1-3 capsid protein set
•    Useful for ratio analysis
•    Precise molar concentration
•    Suitable for WB, dot blot, SDS PAGE

Not available in China and in the Russian Federation.

For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€753.00*

AAV5 VP1, recombinant protein

Cat. No. 640833

•   Recombinant AAV5 capsid protein VP1
•   Useful in combination with AAV5 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72005)
•   Precise molar concentration
•    Suitable for WB, dot blot, SDS PAGE

Not available in China and in the Russian Federation.

For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€291.00*

anti-pan AAV (intact particle) human recombinant, 1C4H

Do the antibodies contain any other proteins?

Can I order bulk amounts of a PROGEN antibody?

Do the PROGEN antibodies contain a preservative?

How can I find the optimal antibody dilution for my experiment?

How are PROGEN antibodies shipped?