Cross reactivity Part A: Dot blot analysis showing the comparison of cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, catalog number 690383) with a multi-serotype recognizing AAV antibody from another vendor. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 1 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to verify binding of the antibody to conformational epitopes. Please note that the two antibody experiments were carried out on separate occasions with different secondary antibodies, which could impact the reliability of direct dot blot comparison.
Cross reactivity Part B: Cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, catalog number 690383) with AAV serotypes and variants, using dot blot analysis. A concentration of 0.2 ug/ml of the anti-pan AAV was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to exclude binding to non-assembled capsids.
Cross reactivity Part A: Dot blot analysis showing the comparison of cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, catalog number 690383) with a multi-serotype recognizing AAV antibody from another vendor. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 1 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to verify binding of the antibody to conformational epitopes. Please note that the two antibody experiments were carried out on separate occasions with different secondary antibodies, which could impact the reliability of direct dot blot comparison.
Cross reactivity Part B: Cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, catalog number 690383) with AAV serotypes and variants, using dot blot analysis. A concentration of 0.2 ug/ml of the anti-pan AAV was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to exclude binding to non-assembled capsids.
Key Features
- Monoclonal recombinant mouse chimeric antibody
- Suitable for dot blot, IF, ELISA and neutralization
- Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAV4A, AAV4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9 intact particles
- Isotype: mouse IgG2a
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Recombinant chimeric (mouse Fc region) |
| Isotype | IgG2a |
| Conjugate | Unconjugated |
| Application | Dot blot, ELISA, ICC/IF, Neutralization assay |
| Purification | Affinity chromatography |
| Reactivity | AAV-F, AAV-LK03, AAV-PHP.eB, AAV1, AAV2, AAV2 7m8, AAV3, AAV4, AAV4A, AAV4E, AAV5, AAV6, AAV7, AAV8, AAV9, AAV9-P5, AAV11, AAV13, AAV Anc80L65, AAVDJ, AAVhu.37, AAV myo, AAVrh10, AAVrh74, scAAV9 |
| Storage | 2-8°C |
| Intended use | Research use only |
| Clone | 1C4M |
| Immunogen | AAV capsids |
| Concentration | 50 µg/ml |
| Formulation | PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:20-1:50 (1-2.5 µg/ml) |
| Dot Blot | 1:500 (0.1 µg/ml; non-denaturing conditions) |
| ELISA | Assay dependent |
| Neutralization Assay | EC50 ~ 7 ng/ml (AAV1), ~ 195 ng/ml (AAV3), ~ 248 ng/ml (AAV5), ~ 8 ng/ml (AAV6), ~ 52 ng/ml (AAV8), ~ 27 ng/ml (AAV9), ~ 441 ng/ml (AAVrh10), assay dependent |
Background
The anti-pan AAV is a recombinant mouse chimeric antibody of human origin, specifically recognizing a conformational epitope only present on fully assembled and intact AAV capsids. It does not detect denatured AAV capsids, making it a versatile tool for various applications, such as tracking the localization of viral particles, studying protein-protein interactions, facilitating AAV particle purification, and determining total AAV capsid titers. Additionally, the antibody exhibits neutralizing activity against multiple AAV serotypes. The anti-pan AAV demonstrates broad binding capabilities across all AAV serotypes, with the exception of AAV12. Furthermore, it binds to numerous additional AAV variants, underscoring its versatility in diverse research and quality control settings.
Limited Use Label License: Research Use Only
Product is exclusively owned by PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
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