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anti-Perilipin 1 (C-terminus) guinea pig polyclonal, serum

The antiserum reacts specifically with perilipin 1 located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue, Leydig cells.
Cat. No.: GP33
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€266.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen C-terminus of perilipin / PLIN1 (hCTA/B; aa 507 - 519; cf. Greenberg et al. 1992, JBC 266, 11341-11346)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 – 1:200
Immunohistochemistry (IHC) - paraffin 1:100 – 1:200 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin / PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kD, respectively.
The antiserum reacts specifically with perilipin / PLIN1 located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. The antiserum does not cross-react with adipophilin (ADRP, also named PLIN2) or TIP47 (also named PLIN3) proteins, or additional members of the PLIN/PAT-family , e.g. MLDP or OXPAT/PAT-1, also named PLIN5, LSDP5.

Learn more about PROGEN Perilipin antibodies.

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