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mouse tongue (courtesy of J.Heß, University Hospital Heidelberg) Zoom

mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Perilipin 2 (N-terminus aa 1-16) guinea pig polyclonal, serum

Specific marker for liposarcoma. Adipophilin/Perilipin 2 is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Detected in the glandular cells of lactating mammary gland, adrenal gland, Sertoli cells, in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; In CD68-positive macrophages. Adipocytes are negative. The antibody recognizes N-terminal aa 1-16 of human adipophilin 2 (PLIN2).
Cat. No.: GP46
Quantity:  100 µL
No. of experiments:  ≤ 300 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

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$356.00
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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide (N-terminal aa 1-16 of human adipophilin / PLIN2)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 300 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - paraffin 1:50 - 1:150 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Adipophilin / ADRP / PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (Heid et al. 1998); inducible by etomoxir.
Enhanced expression of Adipophilin / ADRP / PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study  the virus entry of e.g. HCV via lipid droplets (Hope et al.2002).
Polypeptide reacting: Adipophilin / ADRP / PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.

Tissue immunolocalization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages.
Reactivity on cultured cell lines: PLC.

Hope, R. G., Murphy, D. J. & McLauchlan, J. The Domains Required to Direct Core Proteins of Hepatitis C Virus and GB Virus-B to Lipid Droplets Share Common Features with Plant Oleosin Proteins. J. Biol. Chem. 277, 4261–4270 (2002).
Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309–21 (1998).

Reference

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