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anti-Perilipin 2 (N-terminus aa 6-27) guinea pig polyclonal, serum

Specific marker for liposarcoma. Adipophilin/Perilipin 2 is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Detected in the glandular cells of lactating mammary gland, adrenal gland, Sertoli cells, in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; In CD68-positive macrophages. Adipocytes are negative. The antibody recognizes N-terminal aa 6-27 of human adipophilin 2 (PLIN2).
Cat. No.: GP47
Quantity:  100 µL

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide (N-terminal aa 6-27 of human adipophilin / PLIN2)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Adipophilin / ADRP / PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (Heid et al. 1998; Targett-Adams et al. 2003); inducible by etomoxir.
Enhanced expression of Adipophilin / ADRP / PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (Hope et al. 2002). Polypeptide reacting: Adipophilin / ADRP / PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.

Tissue localization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative.
Also positively stained are lipid-storing CD 68-positive macrophages.

Reactivity on cultured cell lines: PLC.

Targett-Adams, P. et al. Live Cell Analysis and Targeting of the Lipid Droplet-binding Adipocyte Differentiation-related Protein. J. Biol. Chem. 278, 15998–16007 (2003).
Hope, R. G., Murphy, D. J. & McLauchlan, J. The Domains Required to Direct Core Proteins of Hepatitis C Virus and GB Virus-B to Lipid Droplets Share Common Features with Plant Oleosin Proteins. J. Biol. Chem. 277, 4261–4270 (2002).
Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309–21 (1998).

Reference

Reference

PublicationSpeciesApplication
Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013). human WB, ICC-IF

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