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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide of TIP47 C-terminus (hCTA/B; aa 420 - 431); C-terminal epitope |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100 – 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100 – 1:200 (microwave treatment recommended) |
| Western Blot (WB) | 1:1,000 |
Background
Details
TIP47 (tail-interacting protein of 47 kD; also named PLIN3) is involved in lipid droplet maturation (Bulankina et al. 2009). The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. After radical surgery TIP47 serum levels are decreased (Than et al. 1999).
TIP47 shows about 40% sequence homology to adipophilin (PLIN2). The GP32 antiserum, however, is specific for TIP47 (PLIN3) and does not cross-react with adipophilin (PLIN2) and perilipin (PLIN1) or other closely related members of the PLIN/PAT-family.
Learn more about PROGEN Perilipin antibodies.
Bulankina, A. V et al. TIP47 functions in the biogenesis of lipid droplets. J. Cell Biol. 185, 641–55 (2009).
Than, N. G., Sumegi, B., Than, G. N., Kispal, G. & Bohn, H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein. Tumour Biol. J. Int. Soc. Oncodevelopmental Biol. Med. 20, 184–92 (1999).
Reference
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human
WB
ICC-IF
ICC-IF
