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anti-Perilipin 3 (mouse C-Terminus) guinea pig polyclonal, serum

Cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Localized on the surface of lipid droplets present in many cells and tissues, especially in the milk fat globule membranes of human and bovine. Increased amounts are found in cervix carcinoma patients.
Cat. No.: GP37
Quantity:  100 µL
No. of experiments:  ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$333.00
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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide of murine TIP47 C-terminus (aa 424 – 437), C-PFAPGITEKTPEGK
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity mouse, rat (weaker cross-reaction with human)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 – 1:200
Immunohistochemistry (IHC) - paraffin 1:100 – 1:200 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

TIP47 (tail-interacting protein of 47 kD, also named PLIN3) is involved in lipid droplet maturation. The protein has been localized on the surface of lipid droplets present in many cells and tissues, especially in milk fat globule membranes of human and bovine origin.
TIP47 shows about 40% sequence homology to adipophilin. The GP37 antiserum, however, is specific for TIP47/PLIN3 and does not cross-react with adipophilin and perilipin or other proteins of the PLIN/PAT family.
MW 47 262 (calculated from aa sequence data); apparent 52 000 (after SDS-PAGE); pI = 5.45

Reactivity on cultured cell lines: 3T3 and OP9 cells (mouse)

Reference

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