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human skin (courtesy of J.Heß, University Hospital Heidelberg) Zoom

human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Perilipin 3 mouse monoclonal, 49.19, supernatant

The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein and in studies of viral infection via lipid droplet interaction (e.g. Dengue virus).
Cat. No.: 651168
Quantity:  5 mL
No. of experiments:  ≤ 1000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$278.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 49.19
Immunogen Recombinant polypeptide of human TIP47 / PP17 / PLIN3
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 1000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen undiluted - 1:10 (also after fixation with 2% PFA)
Immunohistochemistry (IHC) - paraffin undiluted - 1:10 (microwave treatmtent recommended)
Western Blot (WB) assay dependent (avoid use of milk proteins (e.g. dry milk preparations) in blocking and incubation buffers)

Background

Details

TIP47 (tail-interacting protein of 47 kD; also named Perilipin 3, PLIN3) is involved in lipid droplet maturation (see e.g. Bulankina 2003 and 2009). The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein. Increased amounts of TIP47/PLIN3 are secreted into circulation of cervix carcinoma patients. After radical surgery PLIN3 serum levels are decreased (cf. Than et al. 1999).
TIP47/PLIN3 shows about 40% sequence homology to adipophilin (PLIN2). The antibody, however, is specific for TIP47/PLIN3 and does not cross-react with adipophilin (PLIN2) and perilipin (PLIN1) or other proteins of the PLIN/PAT family.
Reactivity on cultured cell lines: PLC, A431, HT29, HeLa, SV-80; human pre-adipocytes

Bulankina, A. V et al. TIP47 functions in the biogenesis of lipid droplets. J. Cell Biol. 185, 641–55 (2009).
Bulankina, A. V. TIP47 is recruited to lipid droplets and important for the organelle biogenesis and function. Computing (2003).
Than, N. G., Sumegi, B., Than, G. N., Kispal, G. & Bohn, H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): Homology with adipophilin and the mouse adipose, differentiation-related protein. Tumor Biol. 20, 184–192 (1999).

Reference

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