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anti-Perilipin 3 (N-terminus) guinea pig polyclonal, serum

Cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Localized on the surface of lipid droplets present in many cells and tissues, especially in the milk fat globule membranes of human and bovine. Increased amounts are found in cervix carcinoma patients.
Cat. No.: GP30
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€234.00
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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide of TIP47 N-terminus (aa 1 – 16)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID O60664
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 – 1:200
Immunohistochemistry (IHC) - paraffin 1:100 – 1:200 (microwave treatment recommended)
Western Blot (WB) 1:1,000

Background

Details

TIP47 (tail-interacting protein of 47 kD; also named PLIN3) is involved in lipid droplet maturation. The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. After radical surgery TIP47 serum levels are decreased (Than et al. 1999). TIP47 shows about 40% sequence homology to adipophilin (PLIN2). The GP30 antiserum, however, is specific for TIP47 (PLIN3) and does not cross-react with adipophilin (PLIN2) and perilipin (PLIN1) or other proteins of the PLIN/PAT family. Reactivity on cultured cell lines: PLC cells, pretreated with oleic acid.

Learn more about PROGEN Perilipin antibodies.

Than, N. G., Sumegi, B., Than, G. N., Kispal, G. & Bohn, H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein. Tumour Biol. J. Int. Soc. Oncodevelopmental Biol. Med. 20, 184–92 (1999).

Reference

Reference

PublicationSpeciesApplication
Dahlhoff, M. et al. PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo NIH Public Access. Biochim Biophys Acta 1830, 4642–4649 (2013). human WB, IHC (paraffin)
Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013). human WB, ICC-IF
Chang, P., Khatchadourian, A., Anne McKinney, R. & Maysinger, D. Docosahexaenoic acid (DHA): a modulator of microglia activity and dendritic spine morphology. J. Neuroinflammation 12, (2012). mouse WB
Dichlberger, A. et al. Lipid body formation during maturation of human mast cells. J. Lipid Res. 52, 2198–208 (2011). human WB, ICC-IF
Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. an Off. J. United States Can. Acad. Pathol. Inc 23, 480–92 (2010). human WB, IHC
Buers, I. et al. TIP47, a Lipid Cargo Protein Involved in Macrophage Triglyceride Metabolism. Arterioscler. Thromb. Vasc. Biol. 29, 767–773 (2009). human WC, ICC-IF
Cobbe, N. et al. The conserved metalloprotease invadolysin localizes to the surface of lipid droplets. J. Cell Sci. 122, 3414–23 (2009). human WB, ICC-IF
Bulankina, A. V et al. TIP47 functions in the biogenesis of lipid droplets. J. Cell Biol. 185, 641–55 (2009). human WB, ICC-IF
Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–46 (2008). human, bovine WB, IHC
Robenek, H. et al. Butyrophilin controls milk fat globule secretion. Proc. Natl. Acad. Sci. USA 103, 10385–10390 (2006). bovine ICC-IF, IEM
Hänisch, J., Wältermann, M., Robenek, H. & Steinbüchel, A. Eukaryotic Lipid Body Proteins in Oleogenous Actinomycetes and Their Targeting to Intracellular Triacylglycerol Inclusions: Impact on Models of Lipid Body Biogenesis. Appl. Environ. mircrobiology 72, 6743–6750 (2006). human IEM
Muthusamy, K., Halbert, G. & Roberts, F. Immunohistochemical staining for adipophilin, perilipin and TIP47. J Clin Pathol 59, 1166–1170 (2006). human IHC (paraffin)
Robenek, H., Lorkowski, S., Schnoor, M. & Troyer, D. Spatial Integration of TIP47 and Adipophilin in Macrophage Lipid Bodies. J. Biol. Chem. 280, 5789–5794 (2005). human WB, ICC-IF, IEM
Robenek, H., Robenek, M. J. & Troyer, D. PAT family proteins pervade lipid droplet cores. J. Lipid Res. 46, 1331–1338 (2005). human IEM
Robenek, H. et al. Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains. J. Biol. Chem. 280, 26330–26338 (2005). human ICC-IF, IEM

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