human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Perilipin 3 (N-terminus) guinea pig polyclonal, serum

Cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Localized on the surface of lipid droplets present in many cells and tissues, especially in the milk fat globule membranes of human and bovine. Increased amounts are found in cervix carcinoma patients.

Cat. No.: GP30
Quantity: 100 µL
No. of experiments: ≤ 500 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$293.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

SoÅtysik, K. et al. Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis. Nat.Commun. 10, 473 (2019)

human

ICC-IF

Dahlhoff, M. et al. PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo NIH Public Access. Biochim. Biophys. Acta 1830, 4642-4649 (2013).

human

WB
IHC (paraffin)

Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).

human

WB
ICC-IF

Chang, P., Khatchadourian, A., Anne McKinney, R. & Maysinger, D. Docosahexaenoic acid (DHA): a modulator of microglia activity and dendritic spine morphology. J. Neuroinflammation 12, (2012).

mouse

Dichlberger, A. et al. Lipid body formation during maturation of human mast cells. J. Lipid Res. 52, 2198-208 (2011).

human

WB
ICC-IF

Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480-492 (2010).

human

WB
IHC

Buers, I. et al. TIP47, a Lipid Cargo Protein Involved in Macrophage Triglyceride Metabolism. Arterioscler. Thromb. Vasc. Biol. 29, 767-773 (2009).

human

WB
ICC-IF

Cobbe, N. et al. The conserved metalloprotease invadolysin localizes to the surface of lipid droplets. J. Cell Sci. 122, 3414-23 (2009).

human

WB
ICC-IF

Bulankina, A. V et al. TIP47 functions in the biogenesis of lipid droplets. J. Cell Biol. 185, 641-55 (2009).

human

WB
ICC-IF

Robenek, H., Buers, I., Hofnagel, O., Lorkowski, S. & Severs, N. J. GFP-tagged proteins visualized by freeze-fracture immuno-electron microscopy: A new tool in cellular and molecular medicine. J. Cell. Mol. Med. 13, 1381â1390 (2009).

human

WB
ICC-IF

Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936-1946 (2008).

human

WB
IHC

Robenek, H. et al. Butyrophilin controls milk fat globule secretion. Proc. Natl. Acad. Sci. USA 103, 10385-10390 (2006).

bovine

ICC-IF
IEM

HÃ¤nisch, J., WÃ¤ltermann, M., Robenek, H. & SteinbÃ¼chel, A. Eukaryotic Lipid Body Proteins in Oleogenous Actinomycetes and Their Targeting to Intracellular Triacylglycerol Inclusions: Impact on Models of Lipid Body Biogenesis. Appl. Environ. mircrobiology

human

IEM

Muthusamy, K., Halbert, G. & Roberts, F. Immunohistochemical staining for adipophilin, perilipin and TIP47. J. Clin. Pathol. 59, 1166-1170 (2006).

human

IHC (paraffin)

Robenek, H., Robenek, M. J. & Troyer, D. PAT family proteins pervade lipid droplet cores. J. Lipid Res. 46, 1331-1338 (2005).

human

IEM

Robenek, H. et al. Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains*. J. Biol. Chem. 280, 26330-26338 (2005).

human

ICC-IF
IEM

Robenek, H., Lorkowski, S., Schnoor, M. & Troyer, D. Spatial Integration of TIP47 and Adipophilin in Macrophage Lipid Bodies*. J. Biol. Chem. 280, 5789-5794 (2004).

human

WB
ICC-IF
IEM

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
GP30S	anti-Perilipin 3 (N-terminus) guinea pig polyclonal	serum, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70010	anti-Perilipin 1-5 complete sample set		600 µl each antibody	$235.00
70011	anti-Perilipin 2,3,5 sample set		600 µl each antibody	$134.00

Product description

Host	guinea pig
Antibody Type	polyclonal
Immunogen	synthetic peptide of TIP47 N-terminus (aa 1 – 16)
Purification	stabilized antiserum
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide and 0.5% BSA
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID	O60664
No. of experiments	≤ 500 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	bovine, human, mouse, rat

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:100 – 1:250
Immunohistochemistry (IHC) - paraffin	1:100 – 1:250 (microwave treatment recommended)
Western Blot (WB)	1:1,000 - 1:2,000

Background

Details

TIP47 (tail-interacting protein of 47 kD; also named PLIN3) is involved in lipid droplet maturation. The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. After radical surgery TIP47 serum levels are decreased (Than et al. 1999). TIP47 shows about 40% sequence homology to adipophilin (PLIN2). The GP30 antiserum, however, is specific for TIP47 (PLIN3) and does not cross-react with adipophilin (PLIN2) and perilipin (PLIN1) or other proteins of the PLIN/PAT family. Reactivity on cultured cell lines: PLC cells, pretreated with oleic acid.

Learn more about PROGEN Perilipin antibodies.

Than, N. G., Sumegi, B., Than, G. N., Kispal, G. & Bohn, H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein. Tumour Biol. J. Int. Soc. Oncodevelopmental Biol. Med. 20, 184–92 (1999).

Reference

Publication

Species

Application

SoÅtysik, K. et al. Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis. Nat.Commun. 10, 473 (2019)

human

ICC-IF

human

WB
IHC (paraffin)

Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).

human

WB
ICC-IF

Chang, P., Khatchadourian, A., Anne McKinney, R. & Maysinger, D. Docosahexaenoic acid (DHA): a modulator of microglia activity and dendritic spine morphology. J. Neuroinflammation 12, (2012).

mouse

Dichlberger, A. et al. Lipid body formation during maturation of human mast cells. J. Lipid Res. 52, 2198-208 (2011).

human

WB
ICC-IF

Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480-492 (2010).

human

WB
IHC

Buers, I. et al. TIP47, a Lipid Cargo Protein Involved in Macrophage Triglyceride Metabolism. Arterioscler. Thromb. Vasc. Biol. 29, 767-773 (2009).

human

WB
ICC-IF

Cobbe, N. et al. The conserved metalloprotease invadolysin localizes to the surface of lipid droplets. J. Cell Sci. 122, 3414-23 (2009).

human

WB
ICC-IF

Bulankina, A. V et al. TIP47 functions in the biogenesis of lipid droplets. J. Cell Biol. 185, 641-55 (2009).

human

WB
ICC-IF

human

WB
ICC-IF

human

WB
IHC

Robenek, H. et al. Butyrophilin controls milk fat globule secretion. Proc. Natl. Acad. Sci. USA 103, 10385-10390 (2006).

bovine

ICC-IF
IEM

human

IEM

Muthusamy, K., Halbert, G. & Roberts, F. Immunohistochemical staining for adipophilin, perilipin and TIP47. J. Clin. Pathol. 59, 1166-1170 (2006).

human

IHC (paraffin)

Robenek, H., Robenek, M. J. & Troyer, D. PAT family proteins pervade lipid droplet cores. J. Lipid Res. 46, 1331-1338 (2005).

human

IEM

Robenek, H. et al. Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains*. J. Biol. Chem. 280, 26330-26338 (2005).

human

ICC-IF
IEM

Robenek, H., Lorkowski, S., Schnoor, M. & Troyer, D. Spatial Integration of TIP47 and Adipophilin in Macrophage Lipid Bodies*. J. Biol. Chem. 280, 5789-5794 (2004).

human

WB
ICC-IF
IEM

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- By submitting the above request you agree that PROGEN stores and uses the data provided by you in accordance with the terms of use provided in the privacy declaration in order to process your request. You also agree that PROGEN may contact you with regard to your request or for marketing purposes (e.g. Mailchimp-Emailings). You can revoke your permission at any time without giving any reasons by sending an Email to info@progen.com or by writing to PROGEN at Maaßstraße 30, 69123 Heidelberg/Germany.

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anti-Perilipin 3 (N-terminus) guinea pig polyclonal, serum

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