Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
GP30S |
anti-Perilipin 3 (N-terminus) guinea pig polyclonal |
serum, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
70010 |
anti-Perilipin 1-5 complete sample set |
600 µl each antibody |
$235.00
|
|
70011 |
anti-Perilipin 2,3,5 sample set |
600 µl each antibody |
$134.00
|
Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | synthetic peptide of TIP47 N-terminus (aa 1 – 16) |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide and 0.5% BSA |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
UniprotID | O60664 |
No. of experiments | ≤ 500 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | bovine, human, mouse, rat |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | 1:100 – 1:250 |
Immunohistochemistry (IHC) - paraffin | 1:100 – 1:250 (microwave treatment recommended) |
Western Blot (WB) | 1:1,000 - 1:2,000 |
Background
Details
TIP47 (tail-interacting protein of 47 kD; also named PLIN3) is involved in lipid droplet maturation. The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. After radical surgery TIP47 serum levels are decreased (Than et al. 1999). TIP47 shows about 40% sequence homology to adipophilin (PLIN2). The GP30 antiserum, however, is specific for TIP47 (PLIN3) and does not cross-react with adipophilin (PLIN2) and perilipin (PLIN1) or other proteins of the PLIN/PAT family. Reactivity on cultured cell lines: PLC cells, pretreated with oleic acid.
Learn more about PROGEN Perilipin antibodies.
Than, N. G., Sumegi, B., Than, G. N., Kispal, G. & Bohn, H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein. Tumour Biol. J. Int. Soc. Oncodevelopmental Biol. Med. 20, 184–92 (1999).
Reference
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