human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Perilipin 4 (C-terminus) guinea pig polyclonal, serum

S3-12 belongs to the PAT-family proteins, covering the surface of cytoplasmic lipid droplets. S3-12 is positively detected in lipid droplets of cultured cells.

Cat. No.: GP34
Quantity: 100 µL
No. of experiments: ≤ 1000 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$333.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Gallardo-Montejano, V. I. et al. Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1a/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat. Commun. 7, (2016).

mouse

Pourteymour, S. et al. Perilipin 4 in human skeletal muscle: localization and effect of physical activity. Physiol. Rep. 3, (2015).

human

WB
IHC (paraffin)

Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).

human

ICC-IF

Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).

human

Dahlhoff, M. et al. PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo NIH Public Access. Biochim. Biophys. Acta 1830, 4642-4649 (2013).

human

IHC (paraffin)

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
GP34S	anti-Perilipin 4 (C-terminus) guinea pig polyclonal	serum, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70010	anti-Perilipin 1-5 complete sample set		600 µl each antibody	$235.00

Product description

Host	guinea pig
Antibody Type	polyclonal
Immunogen	synthetic peptide (C-terminal aa 1394-1410 of human S3-12 (also named PLIN4); C-QGLEQLLEGLQHNPPLS, coupled to KLH)
Purification	stabilized antiserum
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide and 0.5% BSA
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 1000 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	human

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	1:200 – 1:500
Immunohistochemistry (IHC) - paraffin	1:200 – 1:500 (microwave treatment recommended)
Western Blot (WB)	1:2,000

Background

Details

Polypeptide reacting: S3-12, apparent Mr 140,000 (after SDS-PAGE)

S3-12 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets.

Additional PAT proteins include adipophilin (ADRP/PLIN2), perilipin (PLIN1), TIP47 (PLIN3), and MLDP (OXPAT/PAT-1, PLIN5 or LSDP5) which are expressed in differentiation-related stages of lipid metabolism.

No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.

Tissue Immunolocalization: S3-12 / PLIN4 is positively detected in lipid droplets of cultured cells.

Reactivity on cultured cell lines: PLC (human)

Learn more about PROGEN Perilipin antibodies.

Reference

Publication

Species

Application

Gallardo-Montejano, V. I. et al. Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1a/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat. Commun. 7, (2016).

mouse

Pourteymour, S. et al. Perilipin 4 in human skeletal muscle: localization and effect of physical activity. Physiol. Rep. 3, (2015).

human

WB
IHC (paraffin)

Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).

human

ICC-IF

Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).

human

IHC (paraffin)

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anti-Perilipin 4 (C-terminus) guinea pig polyclonal, serum

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