Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
GP34S |
anti-Perilipin 4 (C-terminus) guinea pig polyclonal |
serum, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
70010 |
anti-Perilipin 1-5 complete sample set |
600 µl each antibody |
$235.00
|
Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | synthetic peptide (C-terminal aa 1394-1410 of human S3-12 (also named PLIN4); C-QGLEQLLEGLQHNPPLS, coupled to KLH) |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide and 0.5% BSA |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 1000 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | human |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:200 – 1:500 |
Immunohistochemistry (IHC) - paraffin | 1:200 – 1:500 (microwave treatment recommended) |
Western Blot (WB) | 1:2,000 |
Background
Details
Polypeptide reacting: S3-12, apparent Mr 140,000 (after SDS-PAGE)
S3-12 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets.
Additional PAT proteins include adipophilin (ADRP/PLIN2), perilipin (PLIN1), TIP47 (PLIN3), and MLDP (OXPAT/PAT-1, PLIN5 or LSDP5) which are expressed in differentiation-related stages of lipid metabolism.
No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.
Tissue Immunolocalization: S3-12 / PLIN4 is positively detected in lipid droplets of cultured cells.
Reactivity on cultured cell lines: PLC (human)
Learn more about PROGEN Perilipin antibodies.
Reference
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IHC (paraffin)