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human skin (courtesy of J.Heß, University Hospital Heidelberg) Zoom

human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Perilipin 4 (C-terminus) guinea pig polyclonal, serum

S3-12 belongs to the PAT-family proteins, covering the surface of cytoplasmic lipid droplets. S3-12 is positively detected in lipid droplets of cultured cells.
highly_published external_validation
Cat. No.: GP34
Quantity:  100 µL

Delivery Time: usually 1-7 working days

$333.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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anti-Perilipin 4 (C-terminus) guinea pig polyclonal

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide (C-terminal aa 1394-1410 of human S3-12 (also named PLIN4); C-QGLEQLLEGLQHNPPLS, coupled to KLH)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:200 – 1:500
Immunohistochemistry (IHC) - paraffin 1:200 – 1:500 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Polypeptide reacting: S3-12, apparent Mr 140,000 (after SDS-PAGE)

S3-12 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets.

Additional PAT proteins include adipophilin (ADRP/PLIN2), perilipin (PLIN1), TIP47 (PLIN3), and MLDP (OXPAT/PAT-1, PLIN5 or LSDP5) which are expressed in differentiation-related stages of lipid metabolism.

No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.

Tissue Immunolocalization: S3-12 / PLIN4 is positively detected in lipid droplets of cultured cells.

Reactivity on cultured cell lines: PLC (human)

Learn more about PROGEN Perilipin antibodies.

Reference

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