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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide (N-terminal aa 1-15 of human S3-12; MQLGSFFGSLPGFSS-C, coupled to KLH) |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:100 – 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100 – 1:200 (microwave treatment recommended) |
| Western Blot (WB) | 1:2,000 |
Background
Details
Polypeptide reacting: S3-12 (also named PLIN4), apparent Mr 140,000 (after SDS-PAGE).
S3-12 pertains to the PLIN (Perilipin)/PAT-family proteins, covering the surface of cytoplasmic lipid droplets.
Additional PAT proteins include adipophilin (ADRP, perilipin 2), TIP47 (perilipin 3), and MLDP (OXPAT/PAT-1 or LSDP5, or perilipin 5) which are expressed in differentiation-related stages of lipid metabolism.
No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.
Tissue Immunolocalization: S3-12 is positively detected in lipid droplets of cultured cells.
Reactivities on cultured cell lines: PLC (human).
Learn more about PROGEN Perilipin antibodies.
Reference
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