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anti-Perilipin 4 (N-terminus) guinea pig polyclonal, serum

S3-12 belongs to the PAT-family proteins, covering the surface of cytoplasmic lipid droplets. S3-12 is positively detected in lipid droplets of cultured cells.
Cat. No.: GP38
Quantity:  100 µL
No. of experiments:  ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$333.00
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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide (N-terminal aa 1-15 of human S3-12; MQLGSFFGSLPGFSS-C, coupled to KLH)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100 – 1:200
Immunohistochemistry (IHC) - paraffin 1:100 – 1:200 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Polypeptide reacting: S3-12 (also named PLIN4), apparent Mr 140,000 (after SDS-PAGE).
S3-12 pertains to the PLIN (Perilipin)/PAT-family proteins, covering the surface of cytoplasmic lipid droplets.
Additional PAT proteins include adipophilin (ADRP, perilipin 2), TIP47 (perilipin 3), and MLDP (OXPAT/PAT-1 or LSDP5, or perilipin 5) which are expressed in differentiation-related stages of lipid metabolism.

No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.

Tissue Immunolocalization: S3-12 is positively detected in lipid droplets of cultured cells.

Reactivities on cultured cell lines: PLC (human).

Learn more about PROGEN Perilipin antibodies.

Reference

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