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WB with anti-Perilipin 5 antibody (Cat. No. 651176, 1:10), human heart lysate Zoom

WB with anti-Perilipin 5 antibody (Cat. No. 651176, 1:10), human heart lysate

anti-Perilipin 5 mouse monoclonal, MLDP-130.336, supernatant

Polypeptide reacting: MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1/Perilipin 5). MLDP pertains to the PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologuous domain called PAT. Additional PAT proteins (perilipins 1 - 5) including adipophilin (ADRP/perilipin 2), perilipin (perilipin 1), and TIP47 (perilipin 3) are expressed in differentiation-related stages of lipid metabolism.
Cat. No.: 651176
Quantity:  5 mL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide); ≤ 10 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$278.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone MLDP-130.336
Immunogen recombinant peptide (aa 1-463), purified from E. coli
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide); ≤ 10 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immuno Electron Microscopy (IEM) assay dependent
Western Blot (WB) 1:10

Background

Details

Polypeptide reacting: MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1 and perilipin 5 / PLIN5), MW 50,844 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE).
MLDP / PLIN5 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologous domain called PAT. Additional PLIN/PAT proteins include adipophilin (ADRP/PLIN2), perilipin (PLIN1), and TIP47 (PLIN3) which are expressed in differentiation-related stages of lipid metabolism.
MLDP / PLIN5 is positively detected in heart and skeletal muscle.

Reactivity on cultured cell lines: Caco-2, PLC

Reference

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