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Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | Phakinin purified from calf lens |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide and 0.5% BSA |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | bovine, human |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | 1:100 |
Western Blot (WB) | 1:5,000 |
Background
Details
Specific detection of phakinin (also described as CP49; Mr 47,000 polypeptide), intermediate filament protein detected in lens.
Specific note: the antiserum cross-reacts with filensin (also described as CP115; Mr 115,000 polypeptide also present in lens).
Reference
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