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anti-Phenylethanolamine-N-Methyltransferase guinea pig polyclonal, serum

Phenylethanolamine-N-methyltransferase (PNMT) converts noradrenaline to adrenaline. The enzyme is present in adrenomedullary cells and in brain neurons. PNMT isolated from bovine adrenal glands.

Cat. No.: 16067
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€245.00
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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Phenylethanolamine-N-methyltransferase from bovine adrenal medulla (Sigma)
Purification stabilized antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 50 - 100 μl dist. water (final solution contains 0.09 % sodium azide, 1% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:400 - 1:800
Immunohistochemistry (IHC) - paraffin 1:200 - 1:400 (microwave treatment recommended)

Background

Details

Phenylethanolamine-N-methyltransferase (PNMT) is an enzyme converting noradrenaline to adrenaline.
The enzyme is present in adrenomedullary cells and in the brain neurons.
Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
Positive control: DEPC-fixed paraffin sections of rat adrenal gland

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