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IHC of rat tongue (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of rat tongue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Plakoglobin mouse monoclonal, PG 5.1, lyophilized, purified

PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions.The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin [identical to gamma-catenin].
highly_published external_validation
Cat. No.: 61005
Quantity:  50 µg
No. of experiments:  ≤ 2000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$251.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone PG 5.1
Immunogen Plakoglobin, "band 5" protein from bovine snout epidermis
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 2000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, chicken, human, mouse, rat, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5
Immunohistochemistry (IHC) - frozen 1:25 - 1:100
Immunohistochemistry (IHC) - paraffin 1:25 - 1:100 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: desmosomes of epithelial and myocardial cells (incl. cultured cells); zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin)

Reactivity on cultured cell lines: Cell cultures forming monolayers

Reference

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