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anti-Plakoglobin mouse monoclonal, PG 5.1, supernatant

PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions.The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin [identical to gamma-catenin].
Cat. No.: 65105
Quantity:  5 mL

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€139.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone PG 5.1
Immunogen Plakoglobin, "band 5" protein from bovine snout epidermis
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, human, mouse, rat, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) ready-to-use
Immunohistochemistry (IHC) - frozen ready-to-use
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: desmosomes of epithelial and myocardial cells (incl. cultured cells); zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to gamma-catenin)

Reactivity on cultured cell lines: Cell cultures forming monolayers.

Reference

Reference

PublicationSpeciesApplication
Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586–608 (2012). mouse WB
Boehnke, K. et al. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur. J. Cell Biol. 86, 731–46 (2007). human IHC (paraffin)
Borrmann, C. M. et al. The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Colocalizations of desmosomal and fascia adhaerens molecules in the intercalated disk. Eur. J. Cell Biol. 85, 469–485 (2006). human, mouse, rat, bovine, eel, carp WB
Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761–774 (2005). human ICC-IF
Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–430 (2005). human WB, IP
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human WB
Caldelari, R. et al. A central role for the armadillo protein plakoglobin in the autoimmune disease pemphigus vulgaris. J. Cell Biol. 153, 823–34 (2001). mouse ICC-IF
Moll, I., Kurzcn, H., Langbein, L., Erner, W. & Franket, W. The Distribution of the DesmosoInal Protein, Plakophilin 1, in Human Skin and Skin Tumors. J. Invest. Dermatol. 108, 139–146 (1997). human IHC (frozen)
Chitaev, N. A. et al. The binding of plakoglobin to desmosomal cadherins: Patterns of binding sites and topogenic potential. J. Cell Biol. 133, 359–369 (1996). human WB, ICC-IF
Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009–1025 (1996). human, bovine IHC (frozen), ICC-IF
Wahl, J. K. et al. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J. Cell Sci. 109 (Pt 5), 1143–1154 (1996). human WB
Achtstätter, T., Fouquet, B., Rungger‐BraUndele, E. & Frnake, W. W. Cytokeratin filaments and desmonsomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation 40, 129–149 (1989). human IHC (frozen)
Cowin, P., Kapprell, H. P., Franke, W. W., Tamkun, J. & Hynes, R. O. Plakoglobin: A protein common to different kinds of intercellular adhering junctions. Cell 46, 1063–1073 (1986). human, bovine WB, ICC-IF, IEM

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