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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | Human recombinant plakophilin 1 |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100 |
| Western Blot (WB) | 1:1,000 |
Background
Details
PP1 antiserum shows distinct suprabasal staining of stratified epithelia, localizing plakophilin 1 in desmosomal plaques and in the nucleus. In Western blot analysis the antiserum reacts specifically with the 80kD plakophilin 1a and 1b polypeptides.
Reactivity on cultured cell lines: BPH-1 (derived from non-neoplastic prostatic tissue).
Reference
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