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anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant

The anti-plakophilin 1 monoclonal antibody recognizes plakophilin 1 in stratified epithelia. Positive reaction with squamous cell carcinoma, negative with adenocarcinoma.
Cat. No.: 65160
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone PP1-5C2
Immunogen plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, pig, Xenopus laevis

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use (preincubation with 0.05 - 0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred)
Immunohistochemistry (IHC) - paraffin ready-to-use (pretreatment with 0.001% trypsin and microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.

Polypeptide reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine).

Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue)

Reference

Reference

  1. Heid HW, Schmidt A, Zimbelmann R, Schäfer S, Winter-Simanowski S, Stumpp S, Keith M, Figge U, Schnölzer M, Franke WW:  Cell type-specific desmosomal plaque proteins of the plakoglobin family: plakophilin 1 ("band 6 protein"). Differentiation, 58:113-131 (1994)
  2. Schmidt A, Heid HW, Schäfer S, Nuber UA, Zimbelmann R, Franke WW:  Desmosomes and cytoskeletal architecture in epithelial differentiation: cell type-specific plaque components and intermediate filament anchorage. Eur J Cell Biol, 65:229-245 (1994).
  3. Schäfer S, Stumpp S, Franke WW: Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation, 60:99-108 (1996)
  4. Moll I, Kurzen H, Langbein L, Franke WW: The distribution of the desmosomal protein, plakophilin 1, in human skin and skin tumors. J Invest Dermatol 108, 139-141 (1997).
  5. Vasioukin V, Bowers E, Bauer C, Degenstein L, Fuchs E: Desmoplakin is essential in epidermal sheet formation. Nature Cell Bio logy 3, 1076-1085 (2001)
  6. South AP, Wan H, Stone MG, Dopping-Hepenstal PJC, Purkis PE, Marshall JF, Leigh IM, Eady RAJ, Hart IR, McGrath JA: Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J Cell Sci 116, 3303-3314 (2003)
  7. Akat K, Mennel H-D, Kremer P, Gassler N, Bleck CKE, Kartenbeck J: Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol 106, 337-347 (2003)
  8. Yang C, Fischer-Kešo R, Schlechter T, Ströbel P, Marx A, Hofmann I: Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumor Biol. (2015) DOI 10.1007/s13277-015-3628-3

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