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anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant

The anti-plakophilin 1 monoclonal antibody recognizes plakophilin 1 in stratified epithelia. Positive reaction with squamous cell carcinoma, negative with adenocarcinoma.
Cat. No.: 65160
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone PP1-5C2
Immunogen plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, pig, Xenopus laevis

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use (preincubation with 0.05 - 0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred)
Immunohistochemistry (IHC) - paraffin ready-to-use (pretreatment with 0.001% trypsin and microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.

Polypeptide reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine).

Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue)

Reference

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